NGFR Antikörper

Produktnummer ABIN1176837

Dieses Anti-NGFR-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NGFR in WB, FACS, IHC (p), IP, ICC, ICFC und MS. Geeignet für Human, Non-Human Primate, Kaninchen, Cat und Frettchen. Dieses Primary Antibody wurde in 4+ Publikationen zitiert.

Kurzübersicht für NGFR Antikörper (ABIN1176837)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reaktivität: Human, Non-Human Primate, Kaninchen, Cat, Frettchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC), Mass Spectrometry (MS)

Klon: NGFR5

Produktdetails anti-NGFR Antikörper

Verwendungszweck: Anti-Hu CD271 Purified

Spezifität: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

Keine Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Human, Non-Human Primates, Feline (Cat), Rabbit, Ferret

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Purified CD271 protein isolated from human melanoma cell line A875

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/mL, Positive tissue: melanoma, heat-mediated antigen retrieval.
Flow cytometry: Extracellular and intracellular staining, recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-NGFR Antikörper (ABIN1176837)

Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, (1994) (PubMed).

Thompson, Schatteman, Gown, Bothwell: "A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue." in: American journal of clinical pathology, Vol. 92, Issue 4, pp. 415-23, (1989) (PubMed).

Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, (1988) (PubMed).

Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." in: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, (1987) (PubMed).

Details zu NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Andere Bezeichnung: CD271

Hintergrund: Nerve growth factor receptor,CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.,TNFRSF16, NGFR, p75NTR, p75NGFR

Gen-ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signalweg, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Growth Factor Binding