EGF Antikörper (Biotin)

Produktnummer ABIN1173136

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EGF in WB, IHC und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EGF Antikörper (Biotin) (ABIN1173136)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-EGF Antikörper (Biotin)

Verwendungszweck: Biotin-Linked Polyclonal Antibody to Epidermal Growth Factor (EGF)

Spezifität: The antibody is a rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: PAA560Hu01Polyclonal Antibody to Epidermal Growth Factor (EGF)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: Epidermal Growth Factor

Hintergrund: URG, Beta-Urogastrone, Pro-epidermal growth factor

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation