Leptin Antikörper

Produktnummer ABIN116132

Der Kaninchen Polyklonal anti-Leptin Antikörper wird verwendet zum Nachweis von Leptin in Proben von Human. Er wurde validiert für WB und EIA.

Kurzübersicht für Leptin Antikörper (ABIN116132)

Target: Leptin (LEP)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Leptin Antikörper

Spezifität: This antibody reacts with Human Leptin.

Aufreinigung: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hLeptin

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhLeptin (7.5 ng/mL), a concentration of 0.5 - 3.0 μg/mL of this antibody is required. ELISA: To detect hLeptin by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 -0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: use at approx. 2 μg/mL for cryostat and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gen-ID: 3952

NCBI Accession: NP_000221

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process