IL1A Antikörper (Biotin)

Produktnummer ABIN116061

Dieses Anti-IL1A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von IL1A in WB und EIA. Geeignet für Human.

Kurzübersicht für IL1A Antikörper (Biotin) (ABIN116061)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL1A Antikörper (Biotin)

Spezifität: This antibody recognizes Human Interleukin-1 alpha (IL-1 alpha).

Aufreinigung: Affinity Chromatography.

Immunogen: Highly pure (>98%) recombinant hIL-1 alpha (human IL-1 alpha).

Anwendungsinformationen

Applikationshinweise: ELISA: To detect hIL-1 alpha by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthIL-1 alpha. Western Blot: To detect hIL-1 alpha by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Centrifuge vial prior to opening! This product is photosensitive and should be protected from light

Lagerung: 4 °C

Informationen zur Lagerung: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: Interleukin-1 alpha / IL-1A

Hintergrund: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gen-ID: 3552

NCBI Accession: NP_000566

UniProt: P01583

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints