IGF1 Antikörper (Biotin)

Produktnummer ABIN116055

Dieser Anti-IGF1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von IGF1 in WB und EIA. Geeignet für Human.

Kurzübersicht für IGF1 Antikörper (Biotin) (ABIN116055)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IGF1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IGF1 Antikörper (Biotin)

Spezifität: This antibody reacts with Insulin-like Growth Factor 1

Aufreinigung: Affinity Chromatography.

Immunogen: E.coli derived recombinant Human IGF-I (Cat#PA071)

Anwendungsinformationen

Applikationshinweise: Direct ELISA: To detect Human IGF-I by direct ELISA (using 100 μL/well antibody solution) aconcentration of approx. 1.0 μg/mL of this antibody is required. This Biotinylated polyclonalantibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Sandwich ELISA: To detect Human IGF-I by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.25-1.0 μg/mL of this antibody is required. Used inconjunction with Polyclonal anti-Human IGF-I (PP1020P1 or PP1020P2) as a Captureantibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Western blot: To detect Human IGF-I by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Hunman IGF-I is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: Insulin-Like Growth Factor I / IGF1

Hintergrund: Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.Synonyms: IBP1, IGF-I, MGF, Mechano growth factor, Somatomedin-C

Gen-ID: 3479

NCBI Accession: NP_001104755

UniProt: P05019

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation