IL1A Antikörper (Biotin)

Produktnummer ABIN115859

Der Kaninchen Polyklonal Anti-IL1A-Antikörper wurde für WB und EIA validiert. Er ist geeignet, IL1A in Proben von Maus zu detektieren.

Kurzübersicht für IL1A Antikörper (Biotin) (ABIN115859)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-IL1A Antikörper (Biotin)

Spezifität: This antibody reacts with Mouse Interleukin-1 alpha (IL-1 alpha).

Aufreinigung: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant mIL-1 alpha

Anwendungsinformationen

Applikationshinweise: ELISA: (Direct): To detect mIL-1α by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2- 0.4 ng/well of recombinant mIL-1α. (Sandwich): To detect mIL-1α by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonalantibody, in conjunction with Polyclonal Anti-Murine IL-1α (PP005P) as a capture antibody,allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1α. Western blot: To detect mIL-1α by Western blot analysis this antibody can be used at a concentration of0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limitfor recombinant mIL-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: Interleukin-1 alpha / IL-1A

Hintergrund: Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Interleukin 1 (IL1), originally known as lymphocyte activating factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin 2. IL1 is primarily released from stimulated macrophages and monocytes, but also is released from several other cell types and is thought to play a key role in inflammatory and immune responses. The two closely related agents, interleukin 1 alpha (IL1 alpha) and interleukin 1 beta (IL1 beta) bind to the same cell surface receptor, elicit nearly identical biological responses and share 25 % homology in their amino acid sequence.Synonyms: Hematopoietin-1, IL-1 alpha, IL1A, IL1F1

Gen-ID: 16175

NCBI Accession: NP_034684

UniProt: P01582

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints