TRAIL Antikörper

Produktnummer ABIN115786

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TRAIL in WB, Func und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TRAIL Antikörper (ABIN115786)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Produktdetails anti-TRAIL Antikörper

Spezifität: Recognises Human TRAIL/APO-II.

Aufreinigung: Affinity Chromatography on Protein A

Immunogen: Recombinant Human TRAIL/APO-II

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a Sandwich ELISA (assuming 100 μL/well), a concentration of 1-2 μg/mL of thisantibody will detect at least 0.2 ng/well of recombinant Human TRAIL/APO-II when usedwith Biotin conjugated anti-Human TRAIL/APO-II (cat. PP1084B) as the detection antibody. Western Blot: To detect Human TRAIL/Apo2L by Western Blot analysis this antibody can beused at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant Human TRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofHuman TRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μg/mL of this antibody isrequired.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Konzentration: 1.0 mg/mL

Buffer: PBS without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Andere Bezeichnung: CD253 / TRAIL

Hintergrund: Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.Synonyms: APO2L, Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10

Gen-ID: 8743

NCBI Accession: NP_003801

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity