E2F1 Antikörper

Produktnummer ABIN115204

Dieser Maus Monoklonal Antikörper detektiert spezifisch E2F1 in WB, IF, IP und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human und Maus.

Kurzübersicht für E2F1 Antikörper (ABIN115204)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E2F1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: KH95

Produktdetails anti-E2F1 Antikörper

Spezifität: This antibody reacts with a protein of 60 kDa.
Cellular Localization: Nuclear.

Aufreinigung: Purified

Immunogen: BALB/C mice were immunized with a recombinant Human E2F-1 protein.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: 1/25-1/50 in an ABCmethod for 30 minutes at room temperature. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil, Breast carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Andere Bezeichnung: E2F1

Hintergrund: E2Fs are DNA-binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 is proposed to be involved in several cellular processes that range from tumor suppressor, cell progression and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1

Gen-ID: 1869

NCBI Accession: NP_005216

UniProt: Q01094

Pathways: p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie