CDC25A Antikörper

Produktnummer ABIN115127

Der Maus Monoklonal anti-CDC25A Antikörper wird verwendet zum Nachweis von CDC25A in Proben von Human und Ratte. Er wurde validiert für WB, IHC (p), IF und IP.

Kurzübersicht für CDC25A Antikörper (ABIN115127)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC25A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: DCS-120-DCS-121

Produktdetails anti-CDC25A Antikörper

Spezifität: This antibody reacts with a 65 kDa protein known as CDC25A.
Cellular Localization: Nuclear membrane.

Aufreinigung: Purified

Immunogen: BALB/C mice were injected with purified recombinant CDC25A.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunflourescence. Immunoprecipitation. Western Blot. Immunohistochemistry on Paraffin Embedded Sections: Use a dilution of 1/50-1/100 in an ABC method (30 minutes at room temperature, requireshigh temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior toimmunostaining)Recommended Positive Control: Tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Andere Bezeichnung: CDC25A

Hintergrund: The activity of cyclin-dependent kinases in cell cycle is regulated by the phosphorylation status, which is controlled by the antagonistic action of wee 1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B and CDC25C. CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S phase and G2 for CDC25B. CDC25C is mainly expressed in G2 phase.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Gen-ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase