MAP2 Antikörper (Biotin)

Produktnummer ABIN111292

Dieser Maus Monoklonal Antikörper detektiert spezifisch MAP2 in WB, IF, IHC (p) und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Rind (Kuh), Huhn, Wachtel und Xenopus laevis.

Kurzübersicht für MAP2 Antikörper (Biotin) (ABIN111292)

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Wachtel, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MAP2 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: AP20

Produktdetails anti-MAP2 Antikörper (Biotin)

Spezifität: Anti-MAP-2 recognizes (Mr 300 kDa)MAP-2 protein from bovine brain. The antibody strongly cross-reacts with MAP-2 from human, rat, mouse, Xenopus, quail and chicken brains. Anti-MAP-2 (Clone AP20) reacts with the high molecular weight forms (2a & 2b) of MAP-2 but not with the low molecular weight form (2c). Anti-MAP-2 reacts with dendrites and cell bodies of neurons.

Aufreinigung: Protein G chromatography

Immunogen: Bovine brain microtubule associated protein 2.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on frozen sections: 1/500. Immunofluorescence: 1/500. WesternBlotting: 1-2 μg/mL for 2hrs at RT). Immunohistology (Formalin/paraffin): 1-2 μg/mL for 30 min at RT. [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mMcitrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. ]Anti-MAP-2 may be used in studies of neuron structure in both normal and malignant braintissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: 0.01 M phosphate buffered saline (PBS) pH 7.2, containing, 1.0 % BSA and 0.09 % sodium azide as a preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for one month or (in small aliquots) at -20 °C for longer.

Details zu MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Andere Bezeichnung: MAP-2

Hintergrund: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a and 2b) and a low molecular weight form (2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.Synonyms: MAP2, Microtubule-associated protein 2, Neuronal Marker

Gen-ID: 4133

NCBI Accession: NP_002365

UniProt: P11137