PCNA Antikörper (AA 111-122)

Produktnummer ABIN1049498

Der Ziege Polyklonal Anti-PCNA-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, PCNA in Proben von Human, Maus, Ratte und Schwein zu detektieren.

Kurzübersicht für PCNA Antikörper (AA 111-122) (ABIN1049498)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PCNA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-PCNA Antikörper

Bindungsspezifität: AA 111-122

Verwendungszweck: PCNA (aa111-122)

Sequenz: EAPNQEKVSD YE

Spezifität: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAPNQEKVSDYE, from the internal region of the protein sequence according to NP_002583.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8 kDa according to Human NP_002583.1). Approx.35 kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33 kDa in Pig Spleen

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung: PCNA

Hintergrund: PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

Gen-ID: 5111, 18538, 25737

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie