TGFB1 Antikörper

Produktnummer ABIN1042684

Produktdetails anti-TGFB1 Antikörper

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schaf, Kaninchen, Nerz

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Reacts with the dimeric (25 kDa) and monomeric (12.5 kDa) forms of TGF-beta under both non-reducing and reducing conditions respectively. This antibody recognizes both human platelet-derived and recombinant TGF-beta in ELISA

Aufreinigung: Protein G purified

Immunogen: TGF-beta from human platelets

Klon: TB21

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Neutralizing: This antibody neutralizes TGF-beta activity in vitro, in an inhibition assay of CCL/64 cell growth and neutralizes the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. IHC: This antibody has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma at a dilution of 1:1000. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blotting: This antibody when used at an antibody concentration of 5--20 ng/mL will allow visualization of 100 ng/lane of TGF-beta.

Kommentare:

Myeloma, fusion partners: Sp2/0 Ag.14

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Whole IgG in 0.01M PBS, pH 7.0

Referenzen für anti-TGFB1 Antikörper (ABIN1042684)

Crawford, Stellmach, Murphy-Ullrich, Ribeiro, Lawler, Hynes, Boivin, Bouck: "Thrombospondin-1 is a major activator of TGF-beta1 in vivo." in: Cell, Vol. 93, Issue 7, pp. 1159-70, (1998) (PubMed).

Details zu TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGF beta1  (TGFB1 Produkte)

Hintergrund: Synonyms:
Camurati Engelmann disease antibody,CED antibody,Diaphyseal dysplasia 1 progressive antibody,DPD1antibody,LAP antibody,Latency-associated peptide antibody,TGF beta 1 antibody,TGF beta antibody,TGF-beta-1antibody,TGF-beta-5 antibody,TGF-beta1 antibody,TGFB 1 antibody,TGFB antibody,Tgfb-1 antibody,TGFB1antibody,TGFB1_HUMAN antibody,TGFbeta antibody,TGFbeta1 antibody,Transforming Growth Factor beta 1antibody,Transforming growth factor, beta 1a antibody

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints