MAPKAP Kinase 2 Antikörper (Internal Region)

Produktnummer ABIN103960

Hersteller Produkt- Nr.: 600-401-281

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MAPKAP Kinase 2 in WB, IHC, ELISA und IP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MAPKAP Kinase 2 Antikörper (Internal Region) (ABIN103960)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Produktdetails anti-MAPKAP Kinase 2 Antikörper

Bindungsspezifität: Internal Region

Hersteller Produkt- Nr.: 600-401-281

Hersteller: Rockland

Verwendungszweck: MAPKAP Kinase 2 Antibody

Kreuzreaktivität (Details): This affinity-purified antibody is directed against rabbit MAPKAP Kinase 2 and is useful in determining its presence in various assays. This polyclonal anti-MAPKAP Kinase 2 antibody recognizes native and over-expressed protein found in various tissues and extracts.

Produktmerkmale: Synonyms: rabbit anti-MAPKAP kinase 2 antibody, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase-2, MK2, mitogen-activated protein kinase-activated protein kinase-2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2

Aufreinigung: Affinity purified antibody

Sterilität: Sterile filtered

Immunogen:

Immunogen: This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of rabbit MAPKAP Kinase 2 conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-MAPKAP kinase 2 antibody is tested by western blotting and suitable for ELISA, immunoprecipitation and kinase assays.  Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. The antibody detects a single 44 kDa protein in crude extracts prepared either from unstimulated (p/n w09-000-364) or TNFα stimulated ((p/n w09-000-367) human HeLa cell lysates. A 1:15,000 dilution is recommended for ELISA. The researcher should determine optimal titers for other applications.

Western Blot Dilution: 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:15,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.9 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Andere Bezeichnung: MAPKAPK2

Hintergrund: Background: MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay.  MK2 is serine-threonine protein kinase that contains a proline rich sequence and two putative SH3-binding sites.  MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The best-characterized MK2 substrate is hsp27. Although unknown, the physiological function of MK2 may be to increase stress resistance.  MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNFα at a post-transcriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.

Gen-ID: 9261

UniProt: P49137

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades