anti-Maus WNT2B Antikörper für Immunocytochemistry

Recommended WNT2B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antikörper
  • wnt2b
  • WNT2B
  • Wnt13
  • WNT13
  • XWNT2
  • XWnt-2
  • Xwnt-2b
  • wnt-2b
  • xwnt2b
  • wingless-type MMTV integration site family, member 2Ba
  • wingless-type MMTV integration site family, member 2B
  • wingless related MMTV integration site 2b
  • wnt2ba
  • WNT2B
  • LOC100227493
  • LOC100349847
  • Wnt2b
  • wnt2b-a
Reaktivität
Human, Maus
64
34
33
13
12
11
11
10
9
8
7
5
2
1
1
1
Wirt
Ziege
64
1
Klonalität
Polyklonal
Konjugat
Dieser WNT2B Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
32
25
14
13
4
2
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN293249
$ 1,123.83
Zzgl. Versandkosten $45.00

General

Antigen Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) Antikörper
Reaktivität Human, Maus
(64), (34), (33), (13), (12), (11), (11), (10), (9), (8), (7), (5), (2), (1), (1), (1)
Wirt Ziege
(64), (1)
Klonalität
Polyklonal
Konjugat Dieser WNT2B Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
(32), (25), (14), (13), (4), (2), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-WNT2B Antikörper

Target Details WNT2B Anwendungsinformationen Handhabung Bilder
Spezifität Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
Reinigung Affinity purified
Immunogen E. coli-derived rmWnt-2b.
Isotyp IgG

Target Details WNT2B

Produktdetails anti-WNT2B Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung WNT2B (WNT2B Antibody Abstract)
Hintergrund Name/Gene ID: WNT2B
Family: WNT

Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of
Gen-ID 7482
UniProt Q93097
Forschungsgebiet Cancer, Wnt, Wnt Signaling
Pathways WNT Signalweg

Anwendungsinformationen

Produktdetails anti-WNT2B Antikörper Target Details WNT2B Handhabung Bilder zurück nach oben
Applikationshinweise Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-WNT2B Antikörper Target Details WNT2B Anwendungsinformationen Bilder zurück nach oben
Format Lyophilized
Rekonstitution deionized water
Konzentration Lot specific
Buffer Lyophilized.