anti-Human E-cadherin Antikörper für Flow Cytometry

Recommended E-cadherin Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antikörper
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • cdh1
  • si:dz180o5.2
  • dZ71B9.1
  • si:dz180o5.1
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • CDH1
  • CDH16
  • HPT-1
  • HPT1
  • arc-1
  • cd324
  • cdhe
  • ecad
  • cad17
  • hpt1
  • cdh16
  • hpt-1
  • LI-cadherin
  • cadherin-17
  • b-cadherin
  • cdh1-a
  • cdhp
  • hjmd
  • pcad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • Arc1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • si:busm1-180o5.2
  • cadherin 1, epithelial
  • cadherin 1
  • si:busm1-180o5.1
  • cadherin 17, LI cadherin (liver-intestine)
  • Cadherin-17
  • cadherin 3, type 1, P-cadherin (placental)
  • E-cadherin-like
  • Aerobic running capacity QTL 1
  • CDH1
  • si:busm1-180o5.2
  • cdh1
  • Cdh1
  • si:busm1-180o5.1
  • CDH17
  • cad17
  • cdh17
  • cdh3-a
  • LOC100356548
  • Arunc1
Epitop
AA 410-460
98
35
35
31
28
19
15
15
15
14
13
10
6
6
6
6
5
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
833
256
145
40
26
16
9
8
8
6
6
4
3
1
1
1
1
1
1
1
1
1
Wirt
Kaninchen
567
273
51
25
2
Klonalität
Polyklonal
Konjugat
Dieser E-cadherin Antikörper ist unkonjugiert
43
40
38
27
24
21
19
19
17
16
15
13
13
13
13
12
12
11
10
10
9
9
6
4
4
3
3
3
3
3
3
2
Applikation
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
650
398
284
215
194
174
164
90
88
37
23
14
12
12
10
7
6
6
4
4
2
2
2
2
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN1387847
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
13.996978 ABIN4899263 CyTOF FACS ICC SimWes WB Goat IgG AA 155-707 Anmelden zum Anzeigen Polyclonal 7
13.996978 ABIN388278 FACS WB Rabbit Ig AA 160-189, N-Term Anmelden zum Anzeigen Polyclonal 3
13.996978 ABIN4895827 FACS Allophycocyanin Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224 1
13.996978 ABIN4895826 FACS Allophycocyanin Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224 1
13.996978 ABIN4895832 FACS PE Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224 3
13.996978 ABIN4895833 FACS PE Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224 3
13.996978 ABIN739460 FACS IF (p) IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 1
13.996978 ABIN4306562 CyTOF FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 Anmelden zum Anzeigen 67A4 1
13.996978 ABIN969036 FACS IHC ELISA WB Mouse IgG1 Anmelden zum Anzeigen 7H12 3
13.996978 ABIN4306576 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 7H12 6
13.996978 ABIN1574190 FACS WB Rabbit IgG AA 650-700 Anmelden zum Anzeigen Polyclonal 3
13.996978 ABIN2659238 FACS PE-Cy7 Rat IgG1 kappa Anmelden zum Anzeigen DECMA-1 7
13.996978 ABIN1106272 EIA FACS IHC (p) WB Mouse IgG1 Anmelden zum Anzeigen 7H12 3
13.996978 ABIN1996052 FACS IF IF/ICC FITC Mouse IgG2a AA 1-707 Anmelden zum Anzeigen 08
13.996978 ABIN1996054 FACS IF IF/ICC PE Mouse IgG2a AA 1-707 Anmelden zum Anzeigen 08
13.996978 ABIN4895830 FACS Alexa Fluor 488 Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224
13.996978 ABIN4895831 FACS Alexa Fluor 488 Mouse IgG2b AA 155-707 Anmelden zum Anzeigen 180224
13.996978 ABIN2657401 CyTOF FACS IF Alexa Fluor 488 Mouse IgG1 kappa Anmelden zum Anzeigen 67A4 7
13.996978 ABIN2657755 FACS Alexa Fluor 647 Mouse IgG1 kappa Anmelden zum Anzeigen 67A4 8
13.996978 ABIN2663365 FACS PE Mouse IgG1 kappa Anmelden zum Anzeigen 67A4 7

General

Antigen Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) Antikörper
Epitop AA 410-460
(98), (35), (35), (31), (28), (19), (15), (15), (15), (14), (13), (10), (6), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(833), (256), (145), (40), (26), (16), (9), (8), (8), (6), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(567), (273), (51), (25), (2)
Klonalität
Polyklonal
Konjugat Dieser E-cadherin Antikörper ist unkonjugiert
(43), (40), (38), (27), (24), (21), (19), (19), (17), (16), (15), (13), (13), (13), (13), (12), (12), (11), (10), (10), (9), (9), (6), (4), (4), (3), (3), (3), (3), (3), (3), (2)
Applikation Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(650), (398), (284), (215), (194), (174), (164), (90), (88), (37), (23), (14), (12), (12), (10), (7), (6), (6), (4), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 4 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-E-cadherin Antikörper

Target Details E-cadherin Anwendungsinformationen Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN1387847) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human E-cadherin
Isotyp IgG

Target Details E-cadherin

Produktdetails anti-E-cadherin Antikörper Anwendungsinformationen Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN1387847) Bilder zurück nach oben
Antigen
Andere Bezeichnung E Cadherin (CDH1 Antibody Abstract)
Hintergrund

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subcellular location: Extracellular

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1

Gen-ID 999
UniProt P12830
Pathways Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

Anwendungsinformationen

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN1387847) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Anwendungsinformationen Referenzen für anti-E-cadherin Antikörper (ABIN1387847) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-E-cadherin Antikörper (ABIN1387847)

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, 2017 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Human).

Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." in: Molecular vision, Vol. 20, pp. 1758-75, 2015

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, 2015

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, 2014

Bilder

Produktdetails anti-E-cadherin Antikörper Target Details E-cadherin Anwendungsinformationen Handhabung Referenzen für anti-E-cadherin Antikörper (ABIN1387847) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E ...
Immunofluorescence (IF) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Immunofluorescence (IF) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD3...
Western Blotting (WB) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at...
Flow Cytometry (FACS) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Mouse splenocytes probed with Rabbit Anti-E cadherin Polyclonal Antibody, PE-Cy5.5 Co...
Flow Cytometry (FACS) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by Independent Validation badge 29768.Histogram of MCF7 cells stained ...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) MCF-7 cells were stained with E cadherin Polyclonal Antibody, Unconjugated at 1:500 i...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. U138 cells probed with Rabbit Ant...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Image provided by One World Lab validation program. A431 cells probed with Rabbit Ant...
'Independent Validation' Siegel
Chargennummer 130902
Validierte Anwendung Flow Cytometry
Positivkontrolle MCF-7 cells
Negativkontrolle SH-SY5Y cells
Bewertung A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.
Primärantikörper
  • Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
  • Catalog number: ABIN1387847
  • Supplier: Anmelden zum Anzeigen
  • Supplier catalog number: Anmelden zum Anzeigen
  • Lot number: 130902
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: MCF-7 cells
  • Negative control: SH-SY5Y cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-Alexa 647 to confirm no background signal produced from secondary antibody alone
Protokoll
  • Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Propidium Iodide (PI) was added to discern live cells from dead cells.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Anmerkungen - The data displayed is gated on PI negative cells.
Validierungsbilder
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 1: Positive control MCF7 cells. The red histogram is unstained cells, the blue...
Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 410-460) antibody (ABIN1387847) Figure 2: Negative control SH-SY5Y cells. The red histogram is unstained cells, the b...