anti-Human MDK Antikörper für ELISA

Recommended MDK Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Midkine (Neurite Growth-Promoting Factor 2) (MDK) Antikörper
  • MK-B
  • PTF-alpha-1
  • mdk
  • midkine
  • negf2
  • MK
  • Mek
  • ARAP
  • NEGF2
  • RIHB
  • midkine (neurite growth-promoting factor 2)
  • midkine
  • mdk-b
  • Mdk
  • MDK
Reaktivität
Human
134
47
39
5
3
2
2
2
2
1
1
Wirt
Kaninchen
127
19
Klonalität
Polyklonal
Konjugat
Dieser MDK Antikörper ist unkonjugiert
19
9
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
120
65
30
13
11
9
9
7
7
6
2
1
1
Hersteller
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Produktnummer ABIN108523
314,29 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
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1 ABIN1993160 ELISA WB FITC Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1993161 ELISA WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2625292 ELISA WB Biotin Mouse IgG AA 22-143 Anmelden zum Anzeigen
1 ABIN2587621 ELISA WB Biotin Rabbit IgG AA 22-143 Anmelden zum Anzeigen Polyclonal
1 ABIN2587626 ELISA WB FITC Mouse IgG AA 22-143 Anmelden zum Anzeigen
1 ABIN2587624 ELISA WB FITC Rabbit IgG AA 22-143 Anmelden zum Anzeigen Polyclonal
1 ABIN2587634 ELISA WB HRP Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2587635 ELISA WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN2587639 ELISA WB Biotin Rabbit IgG Anmelden zum Anzeigen Polyclonal
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1 ABIN2884654 ELISA WB Rabbit IgG AA 94-143 Anmelden zum Anzeigen Polyclonal
1 ABIN601895 IHC ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN223590 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1960563 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
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Applikation / Reaktivität Human
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Enzyme Immunoassay (EIA) 7 Antikörper
Immunochromatography (IC) 1 Antikörper
Immunocytochemistry (ICC) 4 Antikörper
Immunofluorescence (IF) 9 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 5 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper
Immunohistochemistry (IHC) 25 Antikörper
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antikörper
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Immunoprecipitation (IP) 7 Antikörper
Western Blotting (WB) 109 Antikörper

General

Antigen Midkine (Neurite Growth-Promoting Factor 2) (MDK) Antikörper
Reaktivität Human
(134), (47), (39), (5), (3), (2), (2), (2), (2), (1), (1)
Wirt Kaninchen
(127), (19)
Klonalität
Polyklonal
Konjugat Dieser MDK Antikörper ist unkonjugiert
(19), (9), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(120), (65), (30), (13), (11), (9), (9), (7), (7), (6), (2), (1), (1)
Pubmed 3 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDK Antikörper

Target Details MDK Anwendungsinformationen Handhabung ProductDetails: References for anti-MDK antibody (ABIN108523) Bilder
Sequenz The immunization antigen (14.6 kDa) is a protein containing 121 amino acid residues of the human Midkine and 10 additional amino acid residues - HisTag. MKHHHHHHHM KKKDKVKKGG PGSECAEWAW GPCTPSSKDC GVGFREGTCG AQTQRIRCRV PCNWKKEFGA DCKYKFENWG ACDGGTGTKV RQGTLKKARY NAQCQETIRV TKPCTPKTKA KAKAKKGKGK D
Keine Kreuzreaktivität Human
Kreuzreaktivität (Details) Not yet tested in other species.
Reinigung Immunoaffinity chromatography on a column with immobilized recombinant Human Midkine.
Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Human Midkine.
  • Source of Immunogen: E.coli

Target Details MDK

Produktdetails anti-MDK Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-MDK antibody (ABIN108523) Bilder zurück nach oben
Antigen
Andere Bezeichnung Midkine (MDK Antibody Abstract)
Hintergrund
  • Midkine (MK, also called neurite growth promoting factor 2, NEGF-2), a product of a retinoic acid responsive gene, is a secreted 13 kDa protein belonging to the family of heparin binding growth/differen-tiation factors. MK shares 45 % sequence identity with other member of this family called Pleiotrophin (HB-GAM). Midkine is composed of two domains held together by disulfide linkages. The C-terminally located domain contains two heparin binding sites and is usually responsible for midkine activity. Part of the MK activity is enhanced by dimerization of MK. Midkine has been found in vertebrates from human to zebrafish and is most strongly expresed in midgestation. In the adult MK expression is restricted. In addition to normal development, MK is also involved in the pathogenesis of diseases e.g. inflammatory diseases, human carcinomas such as esophageal, stomach, colon, pancreatic, thyroid, lung, urinary, hepatocellular, neuroblastoma, glioblastoma, Wilm´s tumor etc. High MK levels are associated with poor prognosis in some type of cancer. The increased expresion in many carcinomas indicates that MK can be applied to the diagnosis of malignancy. Midkine is expressed during the reparative stage of bone fractures, also supresses infection of certain viruses including HIV in target cells. Anti-apoptotic and cell protecting activity of midkine makes it to be a promissing in therapy.
Forschungsgebiet Neurology
Pathways RTK Signalweg, M Phase, Skeletal Muscle Fiber Development

Anwendungsinformationen

Produktdetails anti-MDK Antikörper Target Details MDK Handhabung ProductDetails: References for anti-MDK antibody (ABIN108523) Bilder zurück nach oben
Applikationshinweise
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDK Antikörper Target Details MDK Anwendungsinformationen ProductDetails: References for anti-MDK antibody (ABIN108523) Bilder zurück nach oben
Format Lyophilized
Rekonstitution Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Konservierungsmittel Azide free
Lagerung 4 °C/-20 °C/-80 °C
Informationen zur Lagerung The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Haltbarkeit See label

ProductDetails: References for anti-MDK antibody (ABIN108523)

Produktdetails anti-MDK Antikörper Target Details MDK Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Shimada H,Nabeya Y,Tagawa M,Okazumi S,Matsubara H,Kadomatsu K,Muramatsu T,Ikematsu S,Sakuma S,Ochiai T: "Preoperative serum midkine concentration is a prognostic marker for esophageal squamous cell carcinoma." in: Cancer Sci, Vol. 94, Issue 7, pp. 628-32, 2003

Ikematsu S,Okamoto K,Yoshida Y,Oda M,Sugano-Nagano H,Ashida K,Kumai H,Kadomatsu K,Muramatsu H,Takashi Muramatsu,Sakuma S: "High levels of urinary midkine in various cancer patients." in: Biochem Biophys Res Commun, Vol. 306, Issue 2, pp. 329-32, 2003

Ikematsu S,Nakagawara A,Nakamura Y,Sakuma S,Wakai K,Muramatsu T,Kadomatsu K: "Correlation of elevated level of blood midkine with poor prognostic factors of human neuroblastomas." in: Br J Cancer, Vol. 88, Issue 10, pp. 1522-6, 2003