Human EPH Receptor A7 ELISA Kit

Recommended EPH Receptor A7 Product (geliefert von: Anmelden zum Anzeigen )

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EPH Receptor A7 (EPHA7) ELISA Kits
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Epitop
pTyr
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
4
3
3
Methodentyp
Sandwich ELISA
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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Produktnummer ABIN4886068
$ 728.20
Zzgl. Versandkosten $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Hersteller References Details
1 ABIN1381110 Cell ELISA Anmelden zum Anzeigen
1 ABIN2113795 Cell ELISA Anmelden zum Anzeigen
1 ABIN2684130 Cell ELISA Cell Samples, Suspension Cell Culture Anmelden zum Anzeigen

General

Antigen EPH Receptor A7 (EPHA7) ELISA Kits
Epitop pTyr
Reaktivität Human
Kits mit alternativen Reaktivitäten:
(4), (3), (3)
Methodentyp Sandwich ELISA
Applikation ELISA
Hersteller Anmelden zum Anzeigen

Produktdetails EPH Receptor A7 ELISA Kit

Antigendetails Anwendungsinformationen Handhabung Bilder
Verwendungszweck Custom Human Phosphotyrosine EphA7 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine EphA7 in lysate samples.
Proben Cell Lysate, Tissue Lysate
Nachweismethode Colorimetric
Analytische Methode Semi-Quantitative
Spezifität The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-EphA7.
Produktmerkmale
  • Rapidly measure phosphorylated protein in lysates
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
Bestandteile
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Biotinylated Anti-Phosphotyrosine Antibody
  • Stop Solution
  • Assay Diluent(s)
  • Positive Control Sample
  • Lysis Buffer
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Benötigtes Material
  • Distilled or deionized water
  • 100 mL and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μL to 1 mL volumes
  • Adjustable 1-25 mL pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Antigendetails

Produktdetails EPH Receptor A7 ELISA Kit Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung EphA7 (EPHA7 ELISA Kit Abstract)
Gen-ID 2045
UniProt Q15375
Pathways RTK Signalweg

Anwendungsinformationen

Produktdetails EPH Receptor A7 ELISA Kit Antigendetails Handhabung Bilder zurück nach oben
Probenmenge 100 μL
Plattentyp Pre-coated
Protokoll
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μL of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails EPH Receptor A7 ELISA Kit Antigendetails Anwendungsinformationen Bilder zurück nach oben
Lagerung -20 °C
Informationen zur Lagerung Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit 6 months