anti-Human RAC1 Antikörper für Immunohistochemistry

Recommended RAC1 Antibody (geliefert von: Anmelden zum Anzeigen )

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Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) Antikörper
  • RAC2
  • ras-related C3 botulinum toxin substrate 1
  • ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
  • Rac1
  • LOC100339873
  • LOC100346252
  • RAC1
Epitop
AA 100-150
15
1
Reaktivität
Human, Ratte (Rattus), Maus
16
15
15
Wirt
Kaninchen
15
1
Klonalität
Polyklonal
Konjugat
Dieser RAC1 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC)
13
4
3
1
Optionen
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Bilder

Produktnummer ABIN350778
$ 454.67
Zzgl. Versandkosten $45.00

General

Antigen Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) Antikörper
Epitop AA 100-150
(15), (1)
Reaktivität Human, Ratte (Rattus), Maus
(16), (15), (15)
Wirt Kaninchen
(15), (1)
Klonalität
Polyklonal
Konjugat Dieser RAC1 Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC)
(13), (4), (3), (1)
Pubmed 8 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-RAC1 Antikörper

Target Details RAC1 Anwendungsinformationen Handhabung Referenzen für anti-RAC1 Antikörper (ABIN350778) Bilder
Spezifität Specific for RAC1 and RAC2.
Reinigung Whole serum
Immunogen A synthetic peptide from aa region 100-150 of of human RAC1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse, guinea pig, bovine, xenopus, dog, zebrafish and chicken.

Target Details RAC1

Produktdetails anti-RAC1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-RAC1 Antikörper (ABIN350778) Bilder zurück nach oben
Antigen
Andere Bezeichnung RAC1, RAC2 (RAC1 Antibody Abstract)
Hintergrund Function: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP, GTPase activating proteins (GAPs) which increase the GTP hydrolysis activity, and GDP dissociation inhibitors which inhibit the dissociation of the nucleotide from the GTPase.
Subcellular location: Cell membrane, Lipid-anchor, Cytoplasmic side. Melanosome. Note: Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. RAC1:
Tissue specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. The expression of isoform B is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues. RAC2:
Tissue specificity: Hematopoietic specific. DISEASE: Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. Also known as: RAC1 is also known as: p21-Rac1, Ras-like protein TC25, Cell migration-inducing gene 5 protein, RAC2 is also known as: p21-Rac2, Small G protein, GX.
Pathways Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-RAC1 Antikörper Target Details RAC1 Handhabung Referenzen für anti-RAC1 Antikörper (ABIN350778) Bilder zurück nach oben
Applikationshinweise A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-RAC1 Antikörper Target Details RAC1 Anwendungsinformationen Referenzen für anti-RAC1 Antikörper (ABIN350778) Bilder zurück nach oben
Format Lyophilized
Rekonstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handhabung Avoid freeze and thaw cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Haltbarkeit 12 months

Referenzen für anti-RAC1 Antikörper (ABIN350778)

Produktdetails anti-RAC1 Antikörper Target Details RAC1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Jezyk, Snyder, Gershberg, Worthylake, Harden, Sondek: "Crystal structure of Rac1 bound to its effector phospholipase C-beta2." in: Nature structural & molecular biology, Vol. 13, Issue 12, pp. 1135-40, 2006

Prehna, Ivanov, Bliska, Stebbins: "Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors." in: Cell, Vol. 126, Issue 5, pp. 869-80, 2006

Sjöblom, Jones, Wood, Parsons, Lin, Barber, Mandelker, Leary, Ptak, Silliman, Szabo, Buckhaults, Farrell, Meeh, Markowitz, Willis, Dawson, Willson, Gazdar, Hartigan, Wu, Liu, Parmigiani, Park et al.: "The consensus coding sequences of human breast and colorectal cancers. ..." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006

Carninci, Kasukawa, Katayama, Gough, Frith, Maeda, Oyama, Ravasi, Lenhard, Wells, Kodzius, Shimokawa, Bajic, Brenner, Batalov, Forrest, Zavolan, Davis, Wilming, Aidinis, Allen, Ambesi-Impiombato et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005

Hillier, Fulton, Fulton, Graves, Pepin, Wagner-McPherson, Layman, Maas, Jaeger, Walker, Wylie, Sekhon, Becker, OLaughlin, Schaller, Fewell, Delehaunty, Miner, Nash, Cordes, Du, Sun, Edwards et al.: "The DNA sequence of human chromosome 7. ..." in: Nature, Vol. 424, Issue 6945, pp. 157-64, 2003

Ambruso, Knall, Abell, Panepinto, Kurkchubasche, Thurman, Gonzalez-Aller, Hiester, deBoer, Harbeck, Oyer, Johnson, Roos: "Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4654-9, 2000

Jordan, Brazåo, Boavida, Gespach, Chastre: "Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors." in: Oncogene, Vol. 18, Issue 48, pp. 6835-9, 2000

Worthylake, Rossman, Sondek: "Crystal structure of Rac1 in complex with the guanine nucleotide exchange region of Tiam1." in: Nature, Vol. 408, Issue 6813, pp. 682-8, 2000

Bilder

Produktdetails anti-RAC1 Antikörper Target Details RAC1 Anwendungsinformationen Handhabung Referenzen für anti-RAC1 Antikörper (ABIN350778) zurück nach oben
Bilder des Herstellers
 image for anti-Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) (AA 100-150) antibody (ABIN350778) IHC on rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 (Ras-like protei...
 image for anti-Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) (AA 100-150) antibody (ABIN350778) IHC on rat sciatic nerve using Rabbit antibody to RAC1, RAC2 (Ras-like protein TC25, ...