Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) protein (T7)

Name: Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42)

Synonyme: G25K, CDC42Hs, AI747189, AU018915, CDC42, MGC128612, Cdc42, MGC86687, zgc:55427, zgc:86687, wu:fi33d02, cdc42, g25k, Xcdc42, cdc42hs, MGC79526, LOC100227983

Hergestellt in: Escherichia Coli (E. coli)

Herkunft (Gen): Human

Konjugat: T7

Applikation: SDS-PAGE (SDS)

Produktnummer: ABIN666665

Produktbeschreibung

Produktmerkmale: CDC42, 1- 188 aa, Human, T7-tagged, Recombinant, E.coli

Format: Liquid

Beschreibung: Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: G25K, Cell division cycle 42 isoform 1 CDC42Hs, Cell division cycle 42, Small GTP binding protein CDC42. NCBI no.: NP_001782

Molekulargewicht: 22.4 kDa (203aa), confirmed by MALDI-TOF.

Anwendungen

Konzentration: 1 mg/ml (determined by Bradford assay)

Reinheit: > 95% by SDS - PAGE

Buffer: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

Lagerung: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

Bilder

Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42) protein (T7)