POLRMT Protein (AA 42-1207) (His tag)

Produktnummer ABIN3136200

Produktdetails

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Protein-Typ: Recombinant

Proteineigenschaft: AA 42-1207

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses POLRMT Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SAASPREQHV LREWGHAELL EVLEARVRQL RAEGTPEMRV KKVQVDRPPQ GHSSRWAQKL EAEKRVKQRR QKEVDQQKQA LTQEFWTLHK EPKIWNKKLA GYLQPSKKGT PTNSEEKQLA QALQAALGRL SSREAEALAR KKAKAVEAQI LVLQQKFLAF FECCVCTGQV PLAHHVLVTH HNNGDRQQVL TLHMYNTVML GWARKGSFRE LVYVFLMLKD AGLSPDLCSY AAALQCMGRR DQDVRTIQRC LKQMMEEGFQ PQLLFTDLVL EEEDRAALLR AVVKAEPAFR PPPQAPSPVN TSTLLKDIYS KEGPVSYPKL HLPLDTLQDL FYQQLHVELS SSVCVQSVEK APVMSKEVIE ARKTLQALRE QWEVELLRVL RETKATMGRQ AYEGQPTLYP FLCLLSEGEF VSILMQVLKV LPAQGEPLIQ LAHNLGLRVL NRHLVKQKQV TNHVQKLGQR YSQYLQLLAS DTQVAPCLPR EYWESLGPLE APAQQPWSVP VLLQLGKQLA ELLVQAVQMP RSLAARQGAQ RSIPVLYHVY SFRSYRQVGI LKPHPAFTHL LETAAEPTLT FETTEVPMLC PPLPWTSLHS GAYLLSSTKL MRATEGTTQH QRLLEQCPPA QLHGPLDALT QLGNCAWRVN GHLLDLVLQI FRDKGCMPLG VPPPRSEAPR PARYQLPPGS TPVHKSELRK ELARCLKVAR EMHSLRSEAL YRLSLAQHLR HRVFWLPHNM DFRGRTYPCP PHFNHLGSDL ARALLEFAEG RPLGPRGLDW LKIHLINLTG LKKGDSLRMR LAFADEVMEE ILDSADNPLT GRKWWMEADE PWQTLACCME VAHAVRSPDP AAYISHLPVH QDGSCNGLQH YAALGRDSVG AASVNLTPSD LPQDVYREVA TQVEEFRQQD AKEGLRVAQV LEGFISRKVV KQTVMTVVYG VTRYGGRLQI EKRLRELSDF PQEFVWEASH YLVRQVFKSL QEMFTSTRAI QHWLTESANL ISHAGWPVEW VTPLGIPIIQ PYHRESKVQV KGGLQSITLT SSVDESQKPN TLKQKNGFPP NFIHSLDSSH MMLTALHCYR KGLIFVSVHD CFWTHAADIP TMNEVCREQF VRLHSQPILE DLAKFLKKRF CSVSSIKSLK SSERALVTKL QETLQSLPKT GTFDLGQVIR STYFFS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Polrmt Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: POLRMT (Polymerase (RNA) Mitochondrial (DNA Directed) (POLRMT))

Andere Bezeichnung: Polrmt (POLRMT Produkte)

Synonyme: polrmt-a Protein, mtRPO Protein, wu:fb73g10 Protein, si:dkeyp-74c5.5 Protein, POLRMT Protein, APOLMT Protein, MTRNAP Protein, MTRPOL Protein, h-mtRPOL Protein, 1110018N15Rik Protein, 4932416K13 Protein, RNA polymerase mitochondrial Protein, polymerase (RNA) mitochondrial (DNA directed) L homeolog Protein, polymerase (RNA) mitochondrial (DNA directed) Protein, Polrmt Protein, polrmt.L Protein, polrmt Protein, POLRMT Protein

Hintergrund: DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates.

Molekulargewicht: 133.3 kDa Including tag.

UniProt: Q8BKF1