VAMP7 Protein (AA 2-188) (His tag)

Produktnummer ABIN3075281

Produktdetails

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-188

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses VAMP7 Protein ist gelabelt mit His tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequenz: AILFAVVARG TTILAKHAWC GGNFLEVTEQ ILAKIPSENN KLTYSHGNYL FHYICQDRIV YLCITDDDFE RSRAFNFLNE IKKRFQTTYG SRAQTALPYA MNSEFSSVLA AQLKHHSENK GLDKVMETQA QVDELKGIMV RNIDLVAQRG ERLELLIDKT ENLVDSSVTF KTTSRNLARA MCMKNLK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VAMP7 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: VAMP7 (Vesicle-Associated Membrane Protein 7 (VAMP7))

Andere Bezeichnung: VAMP7 (VAMP7 Produkte)

Synonyme: zgc:56506 Protein, sybl1 Protein, SYBL1 Protein, TI-VAMP Protein, TIVAMP Protein, VAMP-7 Protein, Sybl1 Protein, synaptobrevin-like 1 Protein, vesicle associated membrane protein 7 L homeolog Protein, vesicle associated membrane protein 7 Protein, vesicle-associated membrane protein 7 Protein, sybl1 Protein, vamp7.L Protein, VAMP7 Protein, Vamp7 Protein

Hintergrund: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. {ECO:0000269|PubMed:10888671, ECO:0000269|PubMed:16677249, ECO:0000269|PubMed:18042464}.

Molekulargewicht: 22.2 kDa Including tag.

UniProt: P51809