Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (Active) protein (His tag)

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Proteinname
  • A630013F22Rik
  • AW552122
  • EPI
  • LACI
  • TFI
  • TFPI1
  • tissue factor pathway inhibitor
  • Tfpi
  • TFPI
Spezies
Human
18
5
3
2
2
2
Quelle
HEK-293 Cells
15
7
3
2
2
2
Protein-Typ
Recombinant
Biologische Aktivität
Active
Aufreinigungstag / Konjugat
Dieses TFPI Protein ist gelabelt mit His tag.
Applikation
Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2. The IC50 value is < 0. 35 nM, as measured in 100 μL reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0. 05 % Brij-35 (w/v), pH 7. 5.

Reinheit >90 % as determined by SDS-PAGE.
Hintergrund Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF), The second K domain inhibits factor Xa, The third K domain binds to heparin, The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.
Molekulargewicht 30 kDa
Gen-ID 7035
NCBI Accession NP_006278
UniProt P10646
Forschungsgebiet Cardiovascular
Applikationshinweise This recombinant protein can be used for WB. For research use only.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4
Lagerung -80 °C,-20 °C
Informationen zur Lagerung Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
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