TFPI Protein (His tag)

Produktnummer ABIN2566958

Produktdetails

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TFPI Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB)

Produktmerkmale:

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca - RPKPVE - Nval - WRK(Dnp) - NH2. The IC50 value is < 0. 35 nM, as measured in 100 μL reaction mixture containing 1. 25 ng trypsin, 10μM substrate, 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0. 05 % Brij-35 (w/v), pH 7. 5.

Reinheit: >90 % as determined by SDS-PAGE.

Anwendungsinformationen

Applikationshinweise: This recombinant protein can be used for WB. For research use only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Lagerung: -80 °C,-20 °C

Informationen zur Lagerung: Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.

Antigendetails

Target: TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))

Andere Bezeichnung: TFPI (TFPI Produkte)

Synonyme: EPI Protein, LACI Protein, TFI Protein, TFPI1 Protein, A630013F22Rik Protein, AW552122 Protein, TFPI Protein, si:dz44f8.1 Protein, tfpib Protein, wu:fb92g01 Protein, zgc:152844 Protein, zgc:158592 Protein, tissue factor pathway inhibitor Protein, tissue factor pathway inhibitor a Protein, TFPI Protein, Tfpi Protein, tfpia Protein

Hintergrund: Tissue factor pathway inhibitor (TFPI) is also known as Extrinsic pathway inhibitor (EPI), Lipoprotein - associated coagulation inhibitor (LACI), is a plasma proteinase inhibitor synthesized by vascular endothelial cells and part of it is associated with glycosaminoglycans of these cells. TFPI is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). TFPI is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region. The first K domain inhibits coagulation factor VIIa complexed to tissue factor (TF), The second K domain inhibits factor Xa, The third K domain binds to heparin, The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces.

Molekulargewicht: 30 kDa

Gen-ID: 7035

NCBI Accession: NP_006278

UniProt: P10646