Recombinant Human NHP2-Like Protein 1/NHP2L1 is produced by our E. coli expression system. The target protein is expressed with sequence (Thr2-Val128) of Human NHP2L1 fused with a His tag at the N-terminus.
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 600 mM NaCl, pH 8.0.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
4 °C/-20 °C/-80 °C
Informationen zur Lagerung
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Haltbarkeit
3 months
Target
NHP2L1
(NHP2 Non-Histone Chromosome Protein 2-Like 1 (NHP2L1))
NHP2-Like Protein 1 (NHP2L1) is a member of the ribosomal protein L7Ae family. NHP2L1 protein is limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 has been shown to interact with RAD17and PRPF31. The protein undergoes a conformational change upon RNA-binding. NHP2L1 binds to the 5-stem-loop of U4 snRNA and may play a role in the late stage of spliceosome assembly, prior to step I of splicing catalysis. Alternative Names: NHP2-Like Protein 1, High Mobility Group-Like Nuclear Protein 2 Homolog 1, OTK27, SNU13 Homolog, hSNU13, U4/U6.U5 tri-snRNP 15.5 kDa Protein, NHP2L1