anti-Maus MLST8 Antikörper für Western Blotting

Recommended MLST8 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8) Antikörper
  • gbl
  • lts8
  • GBL
  • GbetaL
  • LST8
  • POP3
  • WAT1
  • 0610033N12Rik
  • AA409454
  • AI505104
  • AI851821
  • Gbl
  • fi37e04
  • wu:fi37e04
  • zgc:55455
  • zgc:85668
  • MTOR associated protein, LST8 homolog
  • MTOR associated protein, LST8 homolog (S. cerevisiae)
  • mlst8
  • MLST8
  • Mlst8
Reaktivität
Maus
118
64
47
10
9
8
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Wirt
Maus
111
17
2
Klonalität
Monoklonal
Konjugat
Dieser MLST8 Antikörper ist unkonjugiert
7
7
6
5
5
5
1
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
109
44
33
16
13
9
5
3
2
2
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN659124
335,24 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.3140206 ABIN2787309 WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN3198083 IC IF WB Rabbit Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN1873708 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN4313697 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN3021324 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN4313694 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN3024865 WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN2403810 IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN1030409 IHC ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.3140206 ABIN5544953 WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN372413 IHC (p) WB Rabbit IgG AA 150-200 Anmelden zum Anzeigen Polyclonal
1 ABIN575441 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN303264 IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN297122 IHC (p) WB Rabbit AA 150-200 Anmelden zum Anzeigen Polyclonal
1 ABIN5554818 EIA IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN214494 IHC (p) WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1734646 IHC (p) WB Rabbit IgG AA 150-200 Anmelden zum Anzeigen Polyclonal
1 ABIN2470104 IHC WB Rabbit AA 150-200 Anmelden zum Anzeigen Polyclonal
1 ABIN1734645 IHC (p) WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1824743 IP WB Rabbit IgG AA 17-34 Anmelden zum Anzeigen Polyclonal

Ähnliche anti-MLST8 Antikörper

Applikation / Reaktivität Maus
ELISA 12 Antikörper
Enzyme Immunoassay (EIA) 1 Antikörper
Immunochromatography (IC) 1 Antikörper
Immunocytochemistry (ICC) 2 Antikörper
Immunofluorescence (IF) 3 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 1 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antikörper
Immunohistochemistry (IHC) 13 Antikörper
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antikörper
Immunoprecipitation (IP) 5 Antikörper
Proximity Ligation Assay (PLA) 1 Antikörper
Western Blotting (WB) 47 Antikörper

General

Antigen MTOR Associated Protein, LST8 Homolog (S. Cerevisiae) (MLST8) Antikörper
Reaktivität Maus
(118), (64), (47), (10), (9), (8), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Wirt Maus
(111), (17), (2)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser MLST8 Antikörper ist unkonjugiert
(7), (7), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Western Blotting (WB)
(109), (44), (33), (16), (13), (9), (5), (3), (2), (2), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MLST8 Antikörper

Target Details MLST8 Anwendungsinformationen Handhabung ProductDetails: References for anti-MLST8 antibody (ABIN659124) Bilder
Spezifität This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.
Reinigung Ascites
Immunogen This GBL monoclonal antibody is generated from mouse immunized with GBL recombinant protein.
Klon 211CT7-1-1
Isotyp IgG1, kappa

Target Details MLST8

Produktdetails anti-MLST8 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-MLST8 antibody (ABIN659124) Bilder zurück nach oben
Antigen
Andere Bezeichnung GBL (MLST8 Antibody Abstract)
Hintergrund Subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with FRAP1 and enhances its kinase activity. In nutrient-poor conditions, stabilizes the FRAP1-RPTOR interaction and favors RPTOR-mediated inhibition of FRAP1 activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Synonyms: Target of rapamycin complex subunit LST8,MLST8,GBL, LST8
Molekulargewicht 35876 DA
Gen-ID 64223
NCBI Accession NP_071767
UniProt Q9BVC4
Forschungsgebiet Phospho-specific antibodies, Protein Modifications, Cell Structure
Pathways PI3K-Akt Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Autophagie, CXCR4-mediated Signaling Events

Anwendungsinformationen

Produktdetails anti-MLST8 Antikörper Target Details MLST8 Handhabung ProductDetails: References for anti-MLST8 antibody (ABIN659124) Bilder zurück nach oben
Applikationshinweise WB = 1:100-8000
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MLST8 Antikörper Target Details MLST8 Anwendungsinformationen ProductDetails: References for anti-MLST8 antibody (ABIN659124) Bilder zurück nach oben
Format Liquid
Buffer Crude ascites with 0.09 % (W/V) sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung GBL Antibody (ascites) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Haltbarkeit 6 months

ProductDetails: References for anti-MLST8 antibody (ABIN659124)

Produktdetails anti-MLST8 Antikörper Target Details MLST8 Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Ali, Sabatini: "Structure of S6 kinase 1 determines whether raptor-mTOR or rictor-mTOR phosphorylates its hydrophobic motif site." in: The Journal of biological chemistry, Vol. 280, Issue 20, pp. 19445-8, 2005

Jacinto, Loewith, Schmidt, Lin, Rüegg, Hall, Hall: "Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive." in: Nature cell biology, Vol. 6, Issue 11, pp. 1122-8, 2004

Bilder

Produktdetails anti-MLST8 Antikörper Target Details MLST8 Anwendungsinformationen Handhabung ProductDetails: References for anti-MLST8 antibody (ABIN659124) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-MLST8 Antikörper (MTOR Associated Protein, LST8 Homolog (S. Cerevisiae)) (ABIN659124) GBL western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrat...