anti-Affe AKT3 Antikörper für Western Blotting

Recommended AKT3 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) Antikörper
  • AI851531
  • D930002M15Rik
  • Nmf350
  • MPPH
  • PKB-GAMMA
  • PKBG
  • PRKBG
  • RAC-PK-gamma
  • RAC-gamma
  • STK-2
  • Pkbg
  • PKBgamma
  • thymoma viral proto-oncogene 3
  • v-akt murine thymoma viral oncogene homolog 3
  • v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
  • Akt3
  • AKT3
  • akt3
Reaktivität
Human, Affe, Maus, Ratte (Rattus)
298
123
93
14
8
7
6
5
5
5
5
2
1
1
1
1
1
1
Wirt
Kaninchen
164
112
20
2
Klonalität
Polyklonal
Konjugat
Dieser AKT3 Antikörper ist unkonjugiert
13
12
11
10
10
8
5
5
4
4
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
222
157
78
40
34
18
12
11
10
8
8
5
2
2
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

Produktnummer ABIN4902881
$ 323.13
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN213890 IHC (p) WB Mouse IgG1 AA 119-136 Anmelden zum Anzeigen 66C1247-1
1 ABIN481768 IHC (p) ELISA WB Goat AA 119-133 Anmelden zum Anzeigen Polyclonal
1 ABIN230106 Func IP WB Rabbit IgG AA 119-131 Anmelden zum Anzeigen Polyclonal
1 ABIN2615483 ICC IHC (p) WB Rabbit IgG AA 122-136 Anmelden zum Anzeigen Polyclonal
1 ABIN5535461 IHC (p) WB Rabbit Ig Fraction AA 88-118 Anmelden zum Anzeigen Polyclonal
1 ABIN2469638 IHC WB Mouse IgG1 AA 119-136 Anmelden zum Anzeigen 66C1247-1

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) Antikörper
Reaktivität Human, Affe, Maus, Ratte (Rattus)
(298), (123), (93), (14), (8), (7), (6), (5), (5), (5), (5), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(164), (112), (20), (2)
Klonalität
Polyklonal
Konjugat Dieser AKT3 Antikörper ist unkonjugiert
(13), (12), (11), (10), (10), (8), (5), (5), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (IHC), Western Blotting (WB)
(222), (157), (78), (40), (34), (18), (12), (11), (10), (8), (8), (5), (2), (2), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-AKT3 Antikörper

Target Details AKT3 Anwendungsinformationen Handhabung Bilder
Produktmerkmale Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9), and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3α and β (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3β-mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18,19).
Reinigung Affinity purification
Immunogen A synthetic peptide of human AKT3.
Isotyp IgG

Target Details AKT3

Produktdetails anti-AKT3 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung AKT3 (AKT3 Antibody Abstract)
Gen-ID 10000
UniProt Q9Y243
Forschungsgebiet Cancer
Pathways PI3K-Akt Signalweg, RTK Signalweg, TLR Signalweg, Hepatitis C, VEGF Signaling

Anwendungsinformationen

Produktdetails anti-AKT3 Antikörper Target Details AKT3 Handhabung Bilder zurück nach oben
Applikationshinweise WB 1:500-1:2000 , IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-AKT3 Antikörper Target Details AKT3 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 1 mg/mL
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze/thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C.

Bilder

Produktdetails anti-AKT3 Antikörper Target Details AKT3 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3) antibody (ABIN4902881) Western blot analysis of MCF7 cell lysate using AKT3 antibody.