anti-Fledermaus Syntaxin 1A (Brain) Antikörper für Immunohistochemistry (Paraffin-embedded Sections)

Recommended Syntaxin 1A (Brain) Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Syntaxin 1A (Brain) (STX1A) Antikörper
  • HPC-1
  • P35-1
  • STX1
  • SYN1A
  • STX1A
  • hpc-1
  • p35-1
  • stx1
  • sx1a
  • syntaxin1a
  • CG10716
  • CG18615
  • CG31136
  • CG5448
  • CT30033
  • Dm Syx1
  • DmSyx1A
  • Dmel\\CG31136
  • SYX1A
  • Syt1A
  • Syx
  • Syx-1A
  • Syx1
  • Syx1a
  • SyxA
  • anon-EST:Gibbs4
  • anon-WO02059370.54
  • dSyx1
  • l(3)06737
  • synt
  • syt-1A
  • syx
  • syx-1
  • syx-1A
  • syx1
  • syx1A
  • syntaxin 1A
  • syntaxin 1A (brain)
  • Syntaxin 1A
  • syntaxin-1A
  • STX1A
  • Stx1a
  • stx1a
  • Syx1A
  • EDI_005240
  • Tsp_07354
Epitop
Internal Region
46
15
11
10
10
10
9
7
3
2
2
2
2
2
2
1
1
1
1
1
Reaktivität
Fledermaus, Huhn, Rind (Kuh), Hund, Hamster, Human, Affe, Maus, Schwein, Ratte (Rattus)
152
85
73
23
21
17
12
6
4
3
2
2
2
1
1
1
1
1
1
Wirt
Kaninchen
111
44
6
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
5
5
5
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
125
62
54
36
19
19
16
14
13
5
3
1
1
Hersteller
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Bilder

Produktnummer ABIN1049370
$ 451.00
Zzgl. Versandkosten $45.00

General

Antigen Syntaxin 1A (Brain) (STX1A) Antikörper
Epitop Internal Region
(46), (15), (11), (10), (10), (10), (9), (7), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reaktivität Fledermaus, Huhn, Rind (Kuh), Hund, Hamster, Human, Affe, Maus, Schwein, Ratte (Rattus)
(152), (85), (73), (23), (21), (17), (12), (6), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Wirt Kaninchen
(111), (44), (6), (2)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(5), (5), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(125), (62), (54), (36), (19), (19), (16), (14), (13), (5), (3), (1), (1)
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Produktdetails

Antigendetails Anwendungsinformationen Handhabung Bilder
Marke IHC-plus™
Spezifität Human STX1A / Syntaxin 1A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Homologie Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Opossum, Turkey, Chicken (100%) Horse, Xenopus (94%) Gibbon, Sheep, Zebra finch, Salmon, Stickleback, Pufferfish, Zebrafish, Ant, Water flea (88%) Beetle (81%).
Reinigung Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from internal region of human STX1A / Syntaxin 1A. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Pig, Opossum, Turkey, Chicken (100%), Horse, Xenopus (94%), Gibbon, Sheep, Zebra finch, Salmon, Stickleback, Pufferfish, Zebrafish, Ant, Water flea (88%), Beetle (81%).

Type of Immunogen: Synthetic peptide

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung STX1A / Syntaxin 1A (STX1A Antibody Abstract)
Hintergrund Name/Gene ID: STX1A
Family: Syntaxin/epimorphin

Synonyms: STX1A, Syntaxin-1A, p35-1, SYN1a, Syntaxin 1A, Syntaxin 1A (brain), HPC-1, Neuron-specific antigen HPC-1, STX1
Gen-ID 6804
Pathways Peptide Hormone Metabolism, Synaptic Membrane, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport

Anwendungsinformationen

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Applikationshinweise Approved: IHC-P (5 μg/mL)
Kommentare

This antibody has been further validated specifically for use under standardized IHC conditions against formalin-fixed paraffin-embedded human tissues.

Testdurchführung The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
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Handhabung

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Format Liquid
Konzentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails Antigendetails Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Syntaxin 1A (Brain) (STX1A) (Internal Region) antibody (ABIN1049370) Anti-STX1A / Syntaxin 1A antibody ABIN1049370 IHC staining of human brain, cortex. I...