anti-Human MDM2 Antikörper für Immunoprecipitation

Recommended MDM2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
  • ACTFS
  • HDMX
  • hdm2
  • zgc:109834
  • 1700007J15Rik
  • AA415488
  • Mdm-2
  • MDM2 oncogene, E3 ubiquitin protein ligase
  • Mdm2, p53 E3 ubiquitin protein ligase homolog (mouse)
  • p53 E3 ubiquitin protein ligase
  • transformed 3T3 cell double minute 2 homolog (mouse)
  • transformed mouse 3T3 cell double minute 2
  • MDM2
  • mdm2
  • Mdm2
Epitop
Center
46
42
36
29
26
24
15
15
15
12
8
8
7
7
6
5
5
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
379
135
95
14
8
6
4
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
306
77
14
2
Klonalität
Polyklonal
Konjugat
Dieser MDM2 Antikörper ist unkonjugiert
12
12
12
9
9
9
3
3
3
3
3
3
3
3
3
3
3
2
2
Applikation
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
263
137
108
83
71
40
38
24
12
12
12
12
4
4
2
1
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Produkt kostenlos erhalten?

Für Ihre Validierungsdaten erstatten wir Ihnen den vollen Kaufpreis. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Produktnummer ABIN2854782
$ 358.60
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN967511 BI IHC (f) IHC (fro) IP WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 2
1 ABIN180331 IHC (fro) IHC (p) IP WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 3
1 ABIN2957662 IC IF IP IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN214211 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14
1 ABIN2469738 IP IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14
1 ABIN2475548 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14 3
1 ABIN2706519 IC IF IP IHC WB Rabbit pSer166 Anmelden zum Anzeigen Polyclonal
1 ABIN1724157 IHC (fro) IHC (p) IP IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14
1 ABIN1495411 IP Magnetic Particles Rabbit AA 103-119 Anmelden zum Anzeigen Polyclonal
1 ABIN1493454 IP Sepharose Rabbit AA 418-432 Anmelden zum Anzeigen Polyclonal
1 ABIN4904328 IF IHC IP WB Mouse IgG Anmelden zum Anzeigen
1 ABIN2312928 IC IF IP IHC WB Mouse IgG2b kappa AA 26-169 Anmelden zum Anzeigen 3G187
1 ABIN216012 IHC (fro) IF IHC (p) IP WB Mouse IgG2b, kappa AA 26-169 Anmelden zum Anzeigen
1 ABIN2312952 IP IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen 3H2649
1 ABIN2625321 ICC IF IHC (p) IP WB Rabbit pSer166 Anmelden zum Anzeigen Polyclonal
1 ABIN5620135 ICC IF IHC IP WB Rabbit Center Anmelden zum Anzeigen Polyclonal
1 ABIN2313024 IP IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14
1 ABIN5583358 IHC (fro) IHC (p) IP Mouse IgG1 Anmelden zum Anzeigen SMP14
1 ABIN643838 IHC (fro) IHC (rs) IHC (p) IP Mouse IgG1 Anmelden zum Anzeigen
1 ABIN2767770 IHC (fro) IP IHC (p) IHC WB Mouse IgG1 AA 154-167 Anmelden zum Anzeigen SMP14

General

Antigen Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) Antikörper
Epitop Center
(46), (42), (36), (29), (26), (24), (15), (15), (15), (12), (8), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus
(379), (135), (95), (14), (8), (6), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Kaninchen
(306), (77), (14), (2)
Klonalität
Polyklonal
Konjugat Dieser MDM2 Antikörper ist unkonjugiert
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Applikation Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(263), (137), (108), (83), (71), (40), (38), (24), (12), (12), (12), (12), (4), (4), (2), (1), (1), (1), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MDM2 Antikörper

Target Details MDM2 Anwendungsinformationen Handhabung Bilder
Kreuzreaktivität Katze, Hund, Schwein
Kreuzreaktivität (Details) Cat (95 %), Dog (91 %), Pig (93 %)
Produktmerkmale Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
MDM2 antibody
Reinigung Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.
Isotyp IgG

Target Details MDM2

Produktdetails anti-MDM2 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung MDM2 (MDM2 Antibody Abstract)
Hintergrund This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.

Cellular Localization: Nucleus , nucleoplasm , Cytoplasm , nucleolus
Molekulargewicht 55 kDa
Gen-ID 4193
Forschungsgebiet Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie

Anwendungsinformationen

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Handhabung Bilder zurück nach oben
Applikationshinweise Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:1000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:1000*
Kommentare

Positive Control: H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 0.74 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Bilder

Produktdetails anti-MDM2 Antikörper Target Details MDM2 Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg 293T w...
Immunohistochemistry (IHC) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IHC-P Image Immunohistochemical analysis of paraffin-embedded human lung cancer, usin...
Immunofluorescence (IF) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MDM2, ...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg Neuro2...
Western Blotting (WB) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) WB Image MDM2 antibody detects MDM2 protein by western blot analysis. A. 30 μg H1299 ...
Chromatin Immunoprecipitation (ChIP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) ChIP Image Cross-linked ChIP was performed with PC-3 chromatin extract and 5 μg of ei...
Immunoprecipitation (IP) image for anti-Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2) (Center) antibody (ABIN2854782) IP Image MDM2 antibody immunoprecipitates MDM2 protein in IP experiments. IP samples:...