anti-Maus p65 Antikörper für Electrophoretic Mobility-Shift Assay

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
C-Term
136
72
66
58
51
47
43
40
39
36
33
32
25
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
1207
676
518
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Wirt
Kaninchen
1036
147
17
16
11
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
880
429
357
269
224
148
118
88
84
28
21
19
18
11
11
6
6
5
5
4
4
2
2
1
Hersteller
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Produktnummer ABIN5556626
391,60 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN121887 EMSA EIA IHC (fro) IP WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal

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General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop C-Term
(136), (72), (66), (58), (51), (47), (43), (40), (39), (36), (33), (32), (25), (21), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(1207), (676), (518), (73), (59), (55), (31), (30), (28), (25), (15), (8), (8), (6), (5), (4), (2), (1), (1)
Wirt Kaninchen
(1036), (147), (17), (16), (11), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(34), (34), (26), (21), (19), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Electrophoretic Mobility-Shift Assay (EMSA), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(880), (429), (357), (269), (224), (148), (118), (88), (84), (28), (21), (19), (18), (11), (11), (6), (6), (5), (5), (4), (4), (2), (2), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Bilder
Spezifität This antibody may react non-specifically with other proteins. Control Peptide (#R1009CP) will compete only with the specific reaction of antiserum with NFkB p65 (Rel A) subunit.
Produktmerkmale Concentration: 80.0 mg/mL (by Refractometry)
Reinigung Prepared from monospecific antiserum by Delipidation and Defibrination.
Immunogen Human NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH)

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung RELA / NF-kB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-alpha. IkB-alpha binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
Gen-ID 5970
NCBI Accession NP_001138610
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Bilder zurück nach oben
Applikationshinweise Immunoprecipitation, Immunoblotting, ELISA and supershift assays. Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against NFkB p65 (Rel A) at a dilution of 1/2000 to 1/5000 followed by reactionwith Peroxidase conjugated Goat anti-Rabbit IgG (H&L) (Cat#R1364HRP). Anti-NFkB p65 (RelA) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A). This product was also tested in a gel supershift assay and found to be reactive against allp65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 μL per assay. Gel (Super) Shift Information: NFkB gel shift assays are assembled in 20 μL reactions containing 0.28 pmoles NFkB oligoin 10 mM Tris ( pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10 % glycerol. Someprocedures specify the addition of 0.05 % NP-40. When using purified protein, 250-300 ngshould be sufficient to produce a gel shifted complex, while 10 μg HeLa nuclear extract isutilized. The gel shift reactions are then incubated at room temperature for 30 minutes. The
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Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Konzentration 80.0 mg/mL
Lagerung 4 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!