anti-Human p65 Antikörper für Intracellular Staining

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • RELA proto-oncogene, NF-kB subunit
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
pSer529
129
84
66
63
49
44
40
39
38
37
26
26
25
18
17
16
15
15
13
13
13
12
11
10
9
8
7
6
6
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1137
653
506
72
56
55
30
25
25
23
13
8
8
6
4
2
1
1
Wirt
Maus
995
129
17
11
1
1
Klonalität
Monoklonal
Konjugat
Dieser p65 Antikörper ist konjugiert mit PE-Cy7
30
28
22
20
18
18
17
13
12
12
12
8
8
8
8
8
8
8
8
4
4
3
3
2
2
2
2
2
2
Applikation
Intracellular Staining (ICS)
833
429
325
260
199
163
105
90
88
28
19
18
15
12
12
6
6
6
5
5
3
2
2
2
1
Optionen
Hersteller
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Produktnummer ABIN1177097
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1177099 ICS Alexa Fluor 647 Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 2
1 ABIN1177100 ICS Alexa Fluor 488 Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 1
1 ABIN1177098 ICS PE Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 2

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop pSer529
(129), (84), (66), (63), (49), (44), (40), (39), (38), (37), (26), (26), (25), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(1137), (653), (506), (72), (56), (55), (30), (25), (25), (23), (13), (8), (8), (6), (4), (2), (1), (1)
Wirt Maus
(995), (129), (17), (11), (1), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p65 Antikörper ist konjugiert mit PE-Cy7
(30), (28), (22), (20), (18), (18), (17), (13), (12), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2)
Applikation Intracellular Staining (ICS)
(833), (429), (325), (260), (199), (163), (105), (90), (88), (28), (19), (18), (15), (12), (12), (6), (6), (6), (5), (5), (3), (2), (2), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN1177097) Bilder
Marke BD Phosflow™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human NF-kappaB p65 Peptide
Klon K10-895-12-50
Isotyp IgG2b, kappa

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN1177097) Bilder zurück nach oben
Antigen
Andere Bezeichnung NF-kappa B p65 (NFkBP65 Antibody Abstract)
Hintergrund Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
Synonyms: transcription factor p65, RELA, MGC131774, NFKB3
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Referenzen für anti-p65 Antikörper (ABIN1177097) Bilder zurück nach oben
Applikationshinweise This antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD™ Phosflow permeabilization buffers may be used.
Kommentare

BALB/c

Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Referenzen für anti-p65 Antikörper (ABIN1177097) Bilder zurück nach oben
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with PE-Cy7 under optimum conditions, and unconjugated antibody and free PE-Cy7 were removed.

Referenzen für anti-p65 Antikörper (ABIN1177097)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Natoli, Saccani, Bosisio, Marazzi: "Interactions of NF-kappaB with chromatin: the art of being at the right place at the right time." in: Nature immunology, Vol. 6, Issue 5, pp. 439-45, 2005