anti-Human p65 Antikörper für Intracellular Staining

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
pSer529
137
72
65
58
51
47
43
40
39
36
33
32
25
21
18
17
16
15
15
13
13
13
12
11
10
9
9
8
7
6
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1207
677
519
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Wirt
Maus
1037
146
17
16
11
1
Klonalität
Monoklonal
Konjugat
Dieser p65 Antikörper ist konjugiert mit Alexa Fluor 488
34
34
26
21
18
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Intracellular Staining (ICS)
881
429
357
269
224
149
118
88
84
28
22
19
18
11
11
6
6
6
5
4
3
2
2
1
Optionen
Hersteller
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Produktnummer ABIN1177100
252,46 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN1177099 ICS Alexa Fluor 647 Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 2
1 ABIN1177097 ICS PE-Cy7 Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 1
1 ABIN1177098 ICS PE Mouse IgG2b, kappa pSer529 Anmelden zum Anzeigen K10-895-12-50 2

anti-p65 Antikörper für Intracellular Staining mit den meisten Publikationen

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General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop pSer529
(137), (72), (65), (58), (51), (47), (43), (40), (39), (36), (33), (32), (25), (21), (18), (17), (16), (15), (15), (13), (13), (13), (12), (11), (10), (9), (9), (8), (7), (6), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human
(1207), (677), (519), (73), (59), (55), (31), (30), (28), (25), (15), (8), (8), (6), (5), (4), (2), (1), (1)
Wirt Maus
(1037), (146), (17), (16), (11), (1)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser p65 Antikörper ist konjugiert mit Alexa Fluor 488
(34), (34), (26), (21), (18), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Intracellular Staining (ICS)
(881), (429), (357), (269), (224), (149), (118), (88), (84), (28), (22), (19), (18), (11), (11), (6), (6), (6), (5), (4), (3), (2), (2), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN1177100) Bilder
Marke BD Phosflow™
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human NF-kappaB p65 Peptide
Klon K10-895-12-50
Isotyp IgG2b, kappa

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN1177100) Bilder zurück nach oben
Antigen
Andere Bezeichnung NF-kappa B p65 (NFkBP65 Antibody Abstract)
Hintergrund Nuclear factor kB (NF-kB) is a ubiquitously expressed transcription factor that regulates the expression of 200-300 genes. It is crucial for basic cellular responses to stress and pathogens, such as proliferation, survival, development, and apoptosis. The most studied NF-kB complex consists of the p50 (also known as NF-kB1) and p65 (also known as REL-A) subunits, both containing a 300-amino acid region with homology to the Rel proto-oncogene product (RH domain). The RH domain contains motifs for dimerization, nuclear localization, and binding to specific DNA sequences. In addition to the RH domain, the p65 subunit contains the transactivation domain, which is responsible for the interaction with the inhibitor IkB and which contains phosphorylation sites. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkB. This complex prevents nuclear translocation and activity of NF-kB. In response to stimuli such as cytokines, LPS, DNA damage, and viral infections, IkB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkB/NF-kB complex, allowing the free heterodimeric NF-kB to translocate to the nucleus. Furthermore, optimal activation of NF-kB requires phosphorylation in the transactivation domain of p65. In the nucleus, activated NF-kB dimers bind to the kB sites within promoters and enhancers and function as transcriptional activators. The K10-895.12.50 monoclonal antibody recognizes the phosphorylated serine 529 (pS529) in the transactivation domain of human NF-kB p65 subunit.
Synonyms: transcription factor p65, RELA, MGC131774, NFKB3
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung ProductDetails: References for anti-p65 antibody (ABIN1177100) Bilder zurück nach oben
Applikationshinweise This antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used.
Probenmenge 20 μL
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen ProductDetails: References for anti-p65 antibody (ABIN1177100) Bilder zurück nach oben
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

ProductDetails: References for anti-p65 antibody (ABIN1177100)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005