anti-Human p65 Antikörper für Gel Shift

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Reaktivität
Human
1207
677
519
73
59
55
31
30
28
25
15
8
8
6
5
4
2
1
1
Wirt
Kaninchen
1036
147
17
16
11
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
34
34
26
21
19
18
16
16
13
13
13
9
9
9
9
9
9
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Gel Shift (GS)
881
429
357
269
224
149
118
88
84
28
22
18
18
11
11
6
6
6
5
4
4
2
2
1
Optionen
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Produktnummer ABIN105291
412,76 €
Zzgl. Versandkosten 20,00 €, Trockeneispauschale 20,00 € sowie MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN233796 GS WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 5
1 ABIN1043700 GS WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal 8
1 ABIN269735 GS ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN218583 GS IF IHC (p) IP ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1738809 GS IF IHC (p) IP ELISA WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN233522 GS IP ELISA WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN204468 GS ELISA WB Mouse IgG2a C-Term Anmelden zum Anzeigen
1 ABIN233530 GS IHC (fro) IP ELISA WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2877898 GS IP ELISA WB Sheep IgG AA 12-317 Anmelden zum Anzeigen Polyclonal
1 ABIN2475874 GS IF IP ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal 4
1 ABIN285880 GS ELISA WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN209098 GS ICC FACS IHC (fro) IF IHC (p) ELISA WB Mouse IgG3 Anmelden zum Anzeigen
1 ABIN576233 GS IP ELISA WB Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN640572 GS IP ELISA WB Rabbit IgG Anmelden zum Anzeigen
1 ABIN5586929 ELISA GS ICC IF IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN640573 GS IP ELISA WB Sheep IgG Anmelden zum Anzeigen
1 ABIN5586930 ELISA GS WB Rabbit Anmelden zum Anzeigen Polyclonal
1 ABIN5562490 ELISA GS IP WB Sheep IgG Anmelden zum Anzeigen Polyclonal

anti-p65 Antikörper für Gel Shift mit den meisten Publikationen

Ähnliche anti-p65 Antikörper

Applikation / Reaktivität Human
BioImaging (BI) 2 Antikörper
Chromatin Immunoprecipitation (ChIP) 10 Antikörper
Control Peptide (CP) 2 Antikörper
Cytometry by Time of Flight (CyTOF) 4 Antikörper
Dot Blot (DB) 28 Antikörper
Electrophoretic Mobility-Shift Assay (EMSA) 6 Antikörper
ELISA 348 Antikörper
Enzyme Immunoassay (EIA) 22 Antikörper
Flow Cytometry (FACS) 84 Antikörper
Gel Shift (GS) 19 Antikörper
Immunochromatography (IC) 10 Antikörper
Immunocytochemistry (ICC) 85 Antikörper
Immunofluorescence (IF) 222 Antikörper
Immunofluorescence (fixed cells) (IF/ICC) 17 Antikörper
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 118 Antikörper
Immunohistochemistry (IHC) 423 Antikörper

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Reaktivität Human
(1207), (677), (519), (73), (59), (55), (31), (30), (28), (25), (15), (8), (8), (6), (5), (4), (2), (1), (1)
Wirt Kaninchen
(1036), (147), (17), (16), (11), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(34), (34), (26), (21), (19), (18), (16), (16), (13), (13), (13), (9), (9), (9), (9), (9), (9), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Gel Shift (GS)
(881), (429), (357), (269), (224), (149), (118), (88), (84), (28), (22), (18), (18), (11), (11), (6), (6), (6), (5), (4), (4), (2), (2), (1)
Pubmed 8 Publikationen vorhanden
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Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN105291) Bilder
Produktmerkmale Concentration Definition: by UV absorbance at 280 nm
Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Isotyp IgG

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN105291) Bilder zurück nach oben
Antigen
Andere Bezeichnung NFkB p65 (NFkBP65 Antibody Abstract)
Hintergrund NFkB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. Low levels of p52 and p50 homodimers can also exist in cells. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding.  IkB-a binding masks the NFkB nuclear localisation signal (NLS).   A broad range of external stimuli lead to activation of NFkB and set off signalling cascades that ultimately converge on the IkB kinase (IKK) complex. Activated IKK specifically and directly phosphorylates IkB-a and this phophorylation event targets IkB-a for degradation. As a consequence, NFkB NLS is uncovered and nuclear translocation occurs.
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB
Gen-ID 5970, 223468676
UniProt Q04206
Forschungsgebiet Atherosclerosis, Alzheimer's Disease, Apoptosis/Necrosis, Transcription Factors, Innate Immunity
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung ProductDetails: References for anti-p65 antibody (ABIN105291) Bilder zurück nach oben
Applikationshinweise NFkB gel shift assays are assembled in 20µl reactions containing 0.28 pmoles NFkB oligo in 10mM Tris (pH 7.6), 50 mM NaCl, 0.5 mM EDTA, 1.0 mM DTT, 10% glycerol. Some procedures specify the addition of 0.05% NP-40. When using purified protein, 250-300 ng should be sufficient to produce a gel shifted complex, while 10µg HeLa nuclear extract is utilized. The gel shift reactions are then incubated at room temperature for 30 minutes. The complexes are resolved on Tris-Glycine acrylamide gels. Loading dye containing bromophenol blue and xylene cyanol should be added to the negative control reaction only, as these dyes can increase the dissociation of the NFkB complexes. When using HeLa nuclear extract as the source of binding proteins, two sequence-specific gel-shifted complexes are expected, consisting of p50/p50 homodimers and p50/p65 heterodimers. For cells expressing p52, p50, and p65, as many as four sequence-specific gel-shifted complexes could be observed (p52/p52, p50/p50, p52/p65, p50/p65), and if high levels of p65 are present, the p65/p65 homodimer may also be weakly detected. The following reagents have been observed to enhance NFkB binding in vitro: millimolar amounts of GTP and ATP, spermine, spermidine, barium or calcium ions, and µM amounts of Co+3(NH3)6.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen ProductDetails: References for anti-p65 antibody (ABIN105291) Bilder zurück nach oben
Format Liquid
Konzentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C

ProductDetails: References for anti-p65 antibody (ABIN105291)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Graff, Ettayebi, Hardy: "Rotavirus NSP1 inhibits NFkappaB activation by inducing proteasome-dependent degradation of beta-TrCP: a novel mechanism of IFN antagonism." in: PLoS pathogens, Vol. 5, Issue 1, pp. e1000280, 2009

Lord, Savitsky, Sitcheran, Calame, Wright, Ting, Williams: "Blimp-1/PRDM1 mediates transcriptional suppression of the NLR gene NLRP12/Monarch-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2948-58, 2009

Liu, Ju, Willmarth, Casimiro, Ojeifo, Sakamaki, Katiyar, Jiao, Popov, Yu, Wu, Joyce, Wang, Pestell: "Nuclear factor-kappaB enhances ErbB2-induced mammary tumorigenesis and neoangiogenesis in vivo." in: The American journal of pathology, Vol. 174, Issue 5, pp. 1910-20, 2009

Sivaramakrishnan, Niranjali Devaraj: "Morin regulates the expression of NF-kappaB-p65, COX-2 and matrix metalloproteinases in diethylnitrosamine induced rat hepatocellular carcinoma." in: Chemico-biological interactions, Vol. 180, Issue 3, pp. 353-9, 2009

Liu, Li, Khoury, Colgan, Ibla: "Adenosine signaling mediates SUMO-1 modification of IkappaBalpha during hypoxia and reoxygenation." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13686-95, 2009

Kumar, Wu, Collier-Hyams, Kwon, Hanson, Neish: "The bacterial fermentation product butyrate influences epithelial signaling via reactive oxygen species-mediated changes in cullin-1 neddylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 1, pp. 538-46, 2008

Abe, Hines, Zibari, Pavlick, Gray, Kitagawa, Grisham: "Mouse model of liver ischemia and reperfusion injury: method for studying reactive oxygen and nitrogen metabolites in vivo." in: Free radical biology & medicine, Vol. 46, Issue 1, pp. 1-7, 2008

Starkey, Haidacher, LeJeune, Zhang, Tieu, Choudhary, Brasier, Denner, Tilton: "Diabetes-induced activation of canonical and noncanonical nuclear factor-kappaB pathways in renal cortex." in: Diabetes, Vol. 55, Issue 5, pp. 1252-9, 2006

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung ProductDetails: References for anti-p65 antibody (ABIN105291) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (ABIN105291) Immunochemical Rabbit anti-p65 NLS was used at a 1:200 dilution to detect p65 in (A) ...
Immunofluorescence (IF) image for anti-p65 Antikörper (Nuclear Factor-KB P65) (ABIN105291) Immunofluorescencechemistry. antibodies-online's Rabbit anti-p65 NLS was used at a 1:...