anti-Human p65 Antikörper für Flow Cytometry

Recommended p65 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Nuclear Factor-KB P65 (NFkBP65) Antikörper
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Epitop
AA 50-100
129
85
68
64
50
45
41
39
38
38
27
26
25
23
18
18
17
15
15
15
14
13
13
12
11
9
8
7
6
6
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Schwein, Ratte (Rattus)
1169
677
520
71
56
55
30
25
25
23
13
8
8
6
4
2
1
1
Wirt
Kaninchen
1008
140
25
11
1
1
Klonalität
Polyklonal
Konjugat
Dieser p65 Antikörper ist unkonjugiert
30
28
22
21
20
19
16
14
14
14
13
10
10
8
8
8
8
8
8
4
4
3
3
3
2
2
2
2
2
2
1
Applikation
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
844
443
324
259
207
163
106
104
89
28
19
18
15
12
12
8
6
6
5
5
4
2
2
2
1
Optionen
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Produktnummer ABIN668961
$ 307.45
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
14.117655 ABIN252655 CyTOF FACS IHC IHC (p) SimWes WB Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 8
14.117655 ABIN4349897 CyTOF FACS IHC (p) WB Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 4
14.117655 ABIN1882124 FACS IF IHC (p) WB Rabbit AA 10-37, N-Term Anmelden zum Anzeigen Polyclonal 1
14.117655 ABIN1999506 FACS FITC Mouse IgG2a AA 1-306 Anmelden zum Anzeigen 04
14.117655 ABIN4897975 FACS Allophycocyanin Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301
14.117655 ABIN4897976 FACS Alexa Fluor 488 Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301
14.117655 ABIN4897977 FACS PE Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301
14.117655 ABIN4899032 CyTOF FACS Rat IgG2a AA 1-254 Anmelden zum Anzeigen 465003
14.117655 ABIN4900578 CyTOF FACS ICC WB Mouse IgG2b AA 456-551, Isoform 1 Anmelden zum Anzeigen 532301
14.117655 ABIN2658371 FACS APC Mouse IgG2b kappa Anmelden zum Anzeigen 14G10A21 6
14.117655 ABIN2662813 FACS PE Mouse IgG2b kappa Anmelden zum Anzeigen 14G10A21 6
14.117655 ABIN3029810 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 10-37 Anmelden zum Anzeigen Polyclonal
1 ABIN252061 FACS IHC (p) IP WB Biotin Mouse IgG1 kappa Anmelden zum Anzeigen 112A1021 1
1 ABIN1999503 ELISA FACS IF IF/ICC WB Mouse IgG2a AA 1-306 Anmelden zum Anzeigen 04
1 ABIN1874582 FACS IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1723309 FACS IHC (p) WB Mouse IgG1 kappa AA 526-539 Anmelden zum Anzeigen 112A1021
1 ABIN2666383 FACS Rabbit IgG Anmelden zum Anzeigen Polyclonal 4
1 ABIN4349917 FACS Alexa Fluor 647 Rat IgG2a AA 1-254 Anmelden zum Anzeigen 465003
1 ABIN4349915 FACS Alexa Fluor 488 Rat IgG2a AA 1-254 Anmelden zum Anzeigen 465003
1 ABIN4349919 FACS Alexa Fluor 594 Rat IgG2a AA 1-254 Anmelden zum Anzeigen 465003

General

Antigen Nuclear Factor-KB P65 (NFkBP65) Antikörper
Epitop AA 50-100
(129), (85), (68), (64), (50), (45), (41), (39), (38), (38), (27), (26), (25), (23), (18), (18), (17), (15), (15), (15), (14), (13), (13), (12), (11), (9), (8), (7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Schwein, Ratte (Rattus)
(1169), (677), (520), (71), (56), (55), (30), (25), (25), (23), (13), (8), (8), (6), (4), (2), (1), (1)
Wirt Kaninchen
(1008), (140), (25), (11), (1), (1)
Klonalität
Polyklonal
Konjugat Dieser p65 Antikörper ist unkonjugiert
(30), (28), (22), (21), (20), (19), (16), (14), (14), (14), (13), (10), (10), (8), (8), (8), (8), (8), (8), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1)
Applikation Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(844), (443), (324), (259), (207), (163), (106), (104), (89), (28), (19), (18), (15), (12), (12), (8), (6), (6), (5), (5), (4), (2), (2), (2), (1)
Pubmed 15 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-p65 Antikörper

Target Details p65 Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder
Reinigung Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human NFKBp65
Isotyp IgG

Target Details p65

Produktdetails anti-p65 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Antigen
Andere Bezeichnung NFKB p65 (NFkBP65 Antibody Abstract)
Hintergrund

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p15, NFKB1/p5, REL and NFKB2/p52 and the heterodimeric p65-p5 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p5 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Subcellular location: Cytoplasm

Synonyms: p65, NFKB3, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA

Gen-ID 5970
UniProt Q04206
Pathways NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Anwendungsinformationen

Produktdetails anti-p65 Antikörper Target Details p65 Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Applikationshinweise WB 1:100-1000
FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Referenzen für anti-p65 Antikörper (ABIN668961) Bilder zurück nach oben
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months

Referenzen für anti-p65 Antikörper (ABIN668961)

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Ganguly, Wen, Myer, Czech, Sahu, Steinmetz, Raman: "Anti-atherogenic effect of trivalent chromium-loaded CPMV nanoparticles in human aortic smooth muscle cells under hyperglycemic conditions in vitro." in: Nanoscale, Vol. 8, Issue 12, pp. 6542-54, 2016

Yang, Zhong, Li, Xiong, Wang, Peng, Ye: "Hypothermic machine perfusion increases A20 expression which protects renal cells against ischemia/reperfusion injury by suppressing inflammation, apoptosis and necroptosis." in: International journal of molecular medicine, Vol. 38, Issue 1, pp. 161-71, 2016 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rabbit).

Oyagbemi, Omobowale, Asenuga, Adejumobi, Ajibade, Ige, Ogunpolu, Adedapo, Yakubu: "Sodium fluoride induces hypertension and cardiac complications through generation of reactive oxygen species and activation of nuclear factor kappa beta." in: Environmental toxicology, 2016

Xi, Hu, Guo, Wang, Sheng, Han, Yuan, Weng, Xu: "Immunoreactivities of NF-κB, IL-1β and IL-1R in the skin of Chinese brown frog (Rana dybowskii)." in: Acta histochemica, Vol. 119, Issue 1, pp. 64-70, 2016 Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Zheng, Liu, Li, Tang, Zhang, Tang: "Lithium posttreatment confers neuroprotection through glycogen synthase kinase-3β inhibition in intracerebral hemorrhage rats." in: Journal of neurosurgery, pp. 1-9, 2016 (Probematerial (Species): Rat (Rattus)).

Huang, Yang, Wang, Ma, Cui, Huang, Sheng, Weng, Xu: "Immunostimulatory activity of protein hydrolysate from oviductus ranae on macrophage in vitro." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2014, pp. 180234, 2015

Luo, Yang, Tang, Yao, Kong, He, Zhou: "Kaempferol alleviates insulin resistance via hepatic IKK/NF-?B signal in type 2 diabetic rats." in: International immunopharmacology, Vol. 28, Issue 1, pp. 744-50, 2015

Zhang, Wu, Song, Wan, Zhang, Tai, Liu: "Effect of hydrogen-rich water on acute peritonitis of rat models." in: International immunopharmacology, Vol. 21, Issue 1, pp. 94-101, 2014

Niu, Mu, Li, Huang, Yao, Li: "Protective Effects of Chelerythrine Against Lipopolysaccharide-Induced Endotoxic Shock in Mice." in: Inflammation, 2014

Wang, Ma, Su: "NF-?B pathway contributes to cadmium-induced apoptosis of porcine granulosa cells." in: Biological trace element research, Vol. 153, Issue 1-3, pp. 403-10, 2013 (1:100).

Song, Wang, Huang, Xiao, Shen, Xu: "NF ?B expression increases and CFTR and MUC1 expression decreases in the endometrium of infertile patients with hydrosalpinx: a comparative study." in: Reproductive biology and endocrinology : RB&E, Vol. 10, pp. 86, 2013

Lin, Yan, Lin, Zhang, Wang, Yang, Zhang: "SIRT1 regulates TNF-α-induced expression of CD40 in 3T3-L1 adipocytes via NF-κB pathway." in: Cytokine, Vol. 60, Issue 2, pp. 447-55, 2012

Zhao, Huang, Wang, Zhong: "Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin-induced diabetic rats." in: Behavioural brain research, Vol. 238, pp. 44-7, 2012 Von den Autoren verwendete Methode: Western Blotting (WB) (Probematerial (Species): Rat (Rattus)).

Jiang, Tian, Fu, Zhu, Hou: "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." in: European journal of pharmacology, Vol. 640, Issue 1-3, pp. 75-81, 2010

Jing, Piao, Liu, Gao: "Beta2-GPI: a novel factor in the development of hepatocellular carcinoma." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 11, pp. 1671-80, 2010

Bilder

Produktdetails anti-p65 Antikörper Target Details p65 Anwendungsinformationen Handhabung Referenzen für anti-p65 Antikörper (ABIN668961) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (IHC) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin-embedded rat aortic tissue labeled with Rabbit Anti-NFKB ...
Flow Cytometry (FACS) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Mouse splenocytes probed with Rabbit Anti-NFkB p65 Polyclonal Antibody, Unconjugated .
Western Blotting (WB) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) HCCLM3 lysates probed with Anti-NFKB p65 Polyclonal Antibody, Unconjugated at 1:5000...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded rat spleen labeled with Rabbit Anti-NFKB p65 Pol...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Rabbit ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Nuclear Factor-KB P65 (NFkBP65) (AA 50-100) antibody (ABIN668961) Paraformaldehyde-fixed, paraffin embedded rat bladder, Antigen retrieval by boiling i...