anti-Maus MAP1B Antikörper für Immunofluorescence

Recommended MAP1B Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Microtubule-Associated Protein 1B (MAP1B) Antikörper
  • FUTSCH
  • MAP5
  • Mtap1b
  • A230055D22
  • AI843217
  • LC1
  • Mtap-5
  • Mtap5
  • microtubule associated protein 1B
  • microtubule-associated protein 1B
  • MAP1B
  • Map1b
Reaktivität
Rind (Kuh), Huhn, Katze, Human, Maus, Ratte (Rattus)
77
61
54
18
18
17
12
12
2
2
1
Wirt
Maus
65
18
Klonalität
Monoklonal
Konjugat
Dieser MAP1B Antikörper ist unkonjugiert
6
4
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
45
31
28
18
16
15
13
5
5
2
Hersteller
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Bilder

Produktnummer ABIN269612
Preis und Verfügbarkeit auf Anfrage.
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1 ABIN4332595 ICC IF IHC IHC (fro) WB DyLight 550 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332592 ICC IF IHC IHC (fro) WB Biotin Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332593 ICC IF IHC IHC (fro) WB DyLight 650 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332594 ICC IF IHC IHC (fro) WB DyLight 488 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332600 ICC IF IHC IHC (fro) WB Alexa Fluor 488 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332601 ICC IF IHC IHC (fro) WB Alexa Fluor 405 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332602 ICC IF IHC IHC (fro) WB Alexa Fluor 647 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN4332603 ICC IF IHC IHC (fro) WB Alexa Fluor 700 Rabbit C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN1736696 IHC (fro) IF WB Rabbit IgG C-Term, pThr1265 Anmelden zum Anzeigen Polyclonal
1 ABIN2986857 ICC FACS IF IHC WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
1 ABIN1736698 IHC (fro) IF WB DyLight 650 Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1736699 IHC (fro) IF WB DyLight 488 Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1736701 IHC (fro) IF WB DyLight 550 Rabbit IgG C-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Microtubule-Associated Protein 1B (MAP1B) Antikörper
Reaktivität Rind (Kuh), Huhn, Katze, Human, Maus, Ratte (Rattus)
(77), (61), (54), (18), (18), (17), (12), (12), (2), (2), (1)
Wirt Maus
(65), (18)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser MAP1B Antikörper ist unkonjugiert
(6), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (IHC), Microarray (MA), Western Blotting (WB)
(45), (31), (28), (18), (16), (15), (13), (5), (5), (2)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-MAP1B Antikörper

Target Details MAP1B Anwendungsinformationen Handhabung Referenzen für anti-MAP1B Antikörper (ABIN269612) Bilder
Spezifität The antibody does not cross-react with other MAPs or tubulin.
Reinigung Unpurified
Immunogen Rat brain microtubule associated proteins.
Klon AA6
Isotyp IgG1

Target Details MAP1B

Produktdetails anti-MAP1B Antikörper Anwendungsinformationen Handhabung Referenzen für anti-MAP1B Antikörper (ABIN269612) Bilder zurück nach oben
Antigen
Andere Bezeichnung MAP1B (MAP1B Antibody Abstract)
Hintergrund Gene Symbol: MAP1B
Molekulargewicht Theoretical MW: 271 kDa
Gen-ID 4131
UniProt P15205
Pathways Microtubule Dynamics, Regulation of Cell Size

Anwendungsinformationen

Produktdetails anti-MAP1B Antikörper Target Details MAP1B Handhabung Referenzen für anti-MAP1B Antikörper (ABIN269612) Bilder zurück nach oben
Applikationshinweise Western Blot 1:500, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, MicroarrayThe observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protokoll Protocol specific for MAP1B Antibody IHC protocol for cryostat sections (Protocol generously provided by Dr. Yimin Wang, Tissue Regeneration Therapeutics Inc )
1. Take out the cryostat section slides from -80C. Dry for few minutes at RT.
. Rinse slides with PBS to get rid of OCT. 3 min x 2, shake
. Fix slides with 1:1 methanol:acetone for 3 min
. Wash slides with PBS, 3 min x
. Circle tissue sections with PAP-Pen,
. Incubation with Normal Horse Serum: 5 % in 2 %BSA (in PBS-Tween20) for 20 minutes.
. MAP1B anti human antibody (1:200 in 2 % BSA 0.1 % Tween20). Incubate for 1.5 hours at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %), 5min x2The following steps are for IHC only
. Blocking of endogenous peroxidase: Incubation with 0.3 % H2O2 for 10 minutes.
. Wash PBS-Tween (0.1 %), 3 min x
. Incubation with secondary antibody (1:200 with PBS, anti mouse-bio, in AK-5002 kit) for 45 minutes at 37C in a humid chamber.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubate with VECTAINABC reagent solution (bottle C, Cat No: PK6100, contains Avidin-HRP) for 30 minutes at RT in the dark.(For solution C: 10ml PBS + 2 drops of A and 2 drops of B, kept in 4C with dark)Prewarm at RT for 10min before use and drop 2 drops to the sections.
. Wash slides with PBS-Tween (0.1 %) 5min x
. Incubation with peroxidase substrate (Vector Nova Red, SK-4800) until desired stain intensity develops. Usually 1 to 2 minutes.Prepare the substrate immediately before use: can be used for > 1week.- Add 3 drops of Reagent 1 to 5 mL of DDW, mix well- Add 2 drops of Reagent 2 and mix well- Add 2 drops of Reagent 3 and mix well- Add 2 drops of Hydrogen Peroxide Solution and mix well
. Wash slides with tap water for 5 minutes.
. Hematoxylin nuclear staining: (Filter solution when first use, HE staining Jar,2nd one). Stain slides for 5 seconds at room temperature, then carefully rinse for 3 minutes under running cold tap water,
. Mount the slide with cc mount (Sigma, C9368, 30ml)
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-MAP1B Antikörper Target Details MAP1B Anwendungsinformationen Referenzen für anti-MAP1B Antikörper (ABIN269612) Bilder zurück nach oben
Format Liquid
Buffer Ascites
Buffer contains: 15 mM Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid freeze-thaw cycles
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C. Avoid freeze-thaw cycles.

Referenzen für anti-MAP1B Antikörper (ABIN269612)

Produktdetails anti-MAP1B Antikörper Target Details MAP1B Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Kaneko, Arayatrakoollikit, Yamanaka, Ito, Okiji: "Immunohistochemical and gene expression analysis of stem-cell-associated markers in rat dental pulp." in: Cell and tissue research, Vol. 351, Issue 3, pp. 425-32, 2013 (Probematerial (Species): Rat (Rattus)). Weitere Details: Immunohistochemistry

Bilder

Produktdetails anti-MAP1B Antikörper Target Details MAP1B Anwendungsinformationen Handhabung Referenzen für anti-MAP1B Antikörper (ABIN269612) zurück nach oben
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunohistochemistry: MAP1B Antibody (AA6) [ABIN269612] - MAP1B expression on human u...
Immunofluorescence (IF) image for anti-Microtubule-Associated Protein 1B (MAP1B) antibody (ABIN269612) Immunocytochemistry/Immunofluorescence: MAP1B Antibody (AA6) [ABIN269612] - Staining ...