anti-Maus ERK2 Antikörper für Western Blotting

Recommended ERK2 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
  • ATMPK1
  • F14N23.9
  • F14N23_9
  • MITOGEN-ACTIVATED PROTEIN KINASE
  • mitogen-activated protein kinase 1
  • ATMPK2
  • MITOGEN-ACTIVATED PROTEIN KINASE HOMOLOG 2
  • T30E16.13
  • T30E16_13
  • mitogen-activated protein kinase homolog 2
  • ERK2
  • zERK2
  • MAPK1
  • ERK
  • ERT1
  • MAPK2
  • P42MAPK
  • PRKM1
  • PRKM2
  • p38
  • p40
  • p41
  • p41mapk
  • 9030612K14Rik
  • AA407128
  • AU018647
  • C78273
  • Erk2
  • Prkm1
  • p42mapk
  • erk
  • erk2
  • ert1
  • mapk
  • mpk1
  • xp42
  • mapk2
  • prkm1
  • prkm2
  • Xp42
  • mapk1b
  • mapk1
  • mapk1a
  • mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 2
  • mitogen activated protein kinase 1
  • ATMPK1
  • MPK1
  • MPK2
  • MAPK1
  • mapk1
  • Mapk1
  • PfMAP1
  • PVX_084965
  • Tsp_01601
  • mapk1-b
  • mapk1-a
Epitop
N-Term
60
22
15
11
9
9
8
8
5
5
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
351
147
118
50
30
19
14
11
10
9
8
7
5
5
2
2
2
2
2
1
1
1
Wirt
Maus
167
167
17
8
3
Klonalität
Monoklonal
Konjugat
Dieser ERK2 Antikörper ist unkonjugiert
10
9
8
7
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
1
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
324
157
93
82
81
62
50
26
21
9
5
3
3
2
2
1
1
1
1
1
1
1
1
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Produktnummer ABIN126833
418,00 €
Zzgl. Versandkosten 20,00 € und MWSt
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
3.3487515 ABIN1882240 WB Mouse IgG1, kappa AA 154-183 Anmelden zum Anzeigen 814CT5-4-2 5
3.3487515 ABIN126832 EIA IP WB Mouse IgG1 C-Term Anmelden zum Anzeigen 6G11
3.3487515 ABIN967747 IF IP IHC WB Mouse IgG2b AA 219-358 Anmelden zum Anzeigen 33-ERK2 5
3.3487515 ABIN967397 IP WB Mouse IgG1, kappa Anmelden zum Anzeigen G263-7 1
3.3487515 ABIN967748 IF IP IHC WB Mouse IgG2b AA 219-358 Anmelden zum Anzeigen 33-ERK2 5
3.3487515 ABIN1724654 ICC IHC ELISA WB Mouse IgG2a Anmelden zum Anzeigen 4C11 2
3.3487515 ABIN2787870 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN2787871 WB Rabbit Middle Region Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN2788693 WB Rabbit C-Term Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN1107133 IF IHC (p) WB Mouse IgG2a Anmelden zum Anzeigen 4C11 2
3.3487515 ABIN258758 ICC IHC (fro) IF IHC (p) IHC ELISA WB Mouse IgG2a Anmelden zum Anzeigen 4C11
3.3487515 ABIN2706125 WB Rabbit Center Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN1533683 ELISA WB Rabbit IgG Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN3184547 ELISA IF IHC WB Rabbit IgG Internal Region Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN438932 ICC IF IHC IHC (p) IP PLA WB Rabbit AA 310-360 Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN2467812 WB Chicken AA 232-360 Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN4270497 IP WB Rabbit Anmelden zum Anzeigen T8
3.3487515 ABIN3031823 ELISA WB Mouse IgG1, kappa AA 154-183 Anmelden zum Anzeigen 814CT5-4-2
3.3487515 ABIN3020728 IHC WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
3.3487515 ABIN1873622 IF IHC WB Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal

anti-ERK2 Antikörper für Western Blotting mit den meisten Publikationen

Ähnliche anti-ERK2 Antikörper

Applikation / Reaktivität Human Maus Hund Ratte (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 2 Antikörper 1 Antikörper
Simple Western (SimWes) 2 Antikörper
Western Blotting (WB) 314 Antikörper 30 Antikörper 135 Antikörper 112 Antikörper
Proximity Ligation Assay (PLA) 3 Antikörper 2 Antikörper 1 Antikörper 1 Antikörper
Luminex Assay (LMNX) 5 Antikörper
Intracellular Staining (ICS) 3 Antikörper 3 Antikörper
Immunostaining (ISt) 1 Antikörper
Immunoprecipitation (IP) 17 Antikörper 56 Antikörper

General

Antigen Mitogen-Activated Protein Kinase 1 (MAPK1) Antikörper
Epitop N-Term
(60), (22), (15), (11), (9), (9), (8), (8), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(351), (147), (118), (50), (30), (19), (14), (11), (10), (9), (8), (7), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(167), (167), (17), (8), (3)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser ERK2 Antikörper ist unkonjugiert
(10), (9), (8), (7), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1)
Applikation Enzyme Immunoassay (EIA), Western Blotting (WB)
(324), (157), (93), (82), (81), (62), (50), (26), (21), (9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 Publikation vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-ERK2 Antikörper

Target Details ERK2 Anwendungsinformationen Handhabung ProductDetails: References for anti-ERK2 antibody (ABIN126833) Bilder
Spezifität This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).
Produktmerkmale Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2
Reinigung Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
Bestandteile incl. positive Control
Immunogen Peptide conjugated to KLHEpitope: N-terminus
Klon 6H3
Isotyp IgG1

Target Details ERK2

Produktdetails anti-ERK2 Antikörper Anwendungsinformationen Handhabung ProductDetails: References for anti-ERK2 antibody (ABIN126833) Bilder zurück nach oben
Antigen
Andere Bezeichnung ERK2 (MAPK1 Antibody Abstract)
Hintergrund Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK
Molekulargewicht 42 kDa
Gen-ID 5594
UniProt P28482
Forschungsgebiet Stem Cells, Signaling, Inflammation
Pathways MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Anwendungsinformationen

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Handhabung ProductDetails: References for anti-ERK2 antibody (ABIN126833) Bilder zurück nach oben
Applikationshinweise ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protokoll Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen ProductDetails: References for anti-ERK2 antibody (ABIN126833) Bilder zurück nach oben
Rekonstitution Restore with 1.0 mL H2O (15 min, RT).
Buffer 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Haltbarkeit 12 months

ProductDetails: References for anti-ERK2 antibody (ABIN126833)

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, 2008

Bilder

Produktdetails anti-ERK2 Antikörper Target Details ERK2 Anwendungsinformationen Handhabung ProductDetails: References for anti-ERK2 antibody (ABIN126833) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-ERK2 Antikörper (Mitogen-Activated Protein Kinase 1) (N-Term) (ABIN126833) anti-Mitogen-Activated Protein Kinase 1 (MAPK1) (N-Term) antibody