anti-Human Complement Component 5 Antikörper für ELISA

Recommended Complement Component 5 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Complement Component 5 (C5) Antikörper
  • C5
  • LOC565774
  • C5a
  • C5b
  • CPAMD4
  • Hc
  • RGD1561905
  • He
  • complement component 5
  • similar to complement component C5-1
  • hemolytic complement
  • C5
  • LOC565774
  • Hc
Epitop
AA 679-751
24
15
13
6
6
3
2
2
2
1
1
1
1
Reaktivität
Human
145
22
10
7
2
2
2
1
Wirt
Kaninchen
85
76
11
6
Klonalität
Polyklonal
Konjugat
Unkonjugiert
14
12
6
6
5
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA
105
81
28
22
19
16
13
10
10
9
9
7
6
5
4
3
2
2
2
2
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Produktnummer ABIN1997181
$ 218.90
Zzgl. Versandkosten $45.00
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
1 ABIN257905 BP ELISA Func Neut WB Mouse IgG2a Anmelden zum Anzeigen 557 3
1 ABIN1997179 ELISA Rabbit IgG AA 679-751 Anmelden zum Anzeigen Polyclonal 2
1 ABIN2477815 Func ELISA WB Mouse IgG1 Anmelden zum Anzeigen 046-10-2-2 (10-04) 2
1 ABIN1997175 ELISA Mouse IgG2b AA 679-751 Anmelden zum Anzeigen 5E 1D1
1 ABIN560121 ELISA WB Mouse AA 1550-1661, partial Anmelden zum Anzeigen Polyclonal
1 ABIN3029690 FACS IHC ELISA WB Rabbit Ig Fraction AA 253-281 Anmelden zum Anzeigen Polyclonal
1 ABIN215712 IHC (fro) ELISA WB Mouse IgG1 Anmelden zum Anzeigen HCC5-1
1 ABIN2215985 FACS IHC ELISA WB Rabbit IgG AA 253-281, N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2649240 ELISA Rabbit IgG AA 679-751 Anmelden zum Anzeigen 442
1 ABIN2215998 IHC ELISA WB HRP Rabbit IgG AA 253-281, N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2216001 ELISA WB PE Rabbit IgG AA 253-281, N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2215990 ELISA WB APC Rabbit IgG AA 253-281, N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN2215996 ELISA WB FITC Rabbit IgG AA 253-281, N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN4299868 ELISA Func IA Mouse IgG2a N-Term Anmelden zum Anzeigen 561
1 ABIN254197 BP ELISA Neut WB Mouse IgG1 Anmelden zum Anzeigen 39725
1 ABIN206662 Func ELISA WB Mouse IgG1 N-Term Anmelden zum Anzeigen 046-10-2-2 (10-04)
1 ABIN200633 ELISA WB Mouse IgG1, kappa Anmelden zum Anzeigen 12F3
1 ABIN1975587 ELISA WB PE Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1968706 ELISA WB Biotin Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal
1 ABIN1962196 ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Anmelden zum Anzeigen Polyclonal

General

Antigen Complement Component 5 (C5) Antikörper
Epitop AA 679-751
(24), (15), (13), (6), (6), (3), (2), (2), (2), (1), (1), (1), (1)
Reaktivität Human
(145), (22), (10), (7), (2), (2), (2), (1)
Wirt Kaninchen
(85), (76), (11), (6)
Klonalität
Polyklonal
Konjugat Unkonjugiert
(14), (12), (6), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation ELISA
(105), (81), (28), (22), (19), (16), (13), (10), (10), (9), (9), (7), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails

Antigendetails Anwendungsinformationen Handhabung Referenzen Bilder
Spezifität Human Complement Component C5a / C5a
Produktmerkmale Produced in rabbits immunized with purified, recombinant Human C5a (rh C5a, ABIN2003124 Leu 679-Arg 751,. C5a specific IgG was purified by human C5a affinity chromatography .
Reinigung purified by affinity chromatography
Sterilität 0.2 μm filtered
Immunogen Recombinant Human C5a protein (ABIN2003124)
Isotyp IgG

Antigendetails

Produktdetails Anwendungsinformationen Handhabung Referenzen Bilder zurück nach oben
Antigen
Hintergrund C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.
Forschungsgebiet Innate Immunity, Complement Factors
Pathways Komplementsystem, Carbohydrate Homeostasis

Anwendungsinformationen

Produktdetails Antigendetails Handhabung Referenzen Bilder zurück nach oben
Applikationshinweise ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human C5a. The detection limit for Human C5a is approximately 0.00975 ng/well.
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails Antigendetails Anwendungsinformationen Referenzen Bilder zurück nach oben
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Konservierungsmittel Without preservative
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C,-20 °C,-80 °C
Informationen zur Lagerung This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Haltbarkeit 12 months

Referenzen

Produktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Allgemeine Veröffentlichungen

Manthey, Woodruff, Taylor, Monk: "Complement component 5a (C5a)." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 11, pp. 2114-7, 2009

Guo, Ward: "Role of C5a in inflammatory responses." in: Annual review of immunology, Vol. 23, pp. 821-52, 2005