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|Antigen||Complement Component 5 (C5) Antikörper|
|Epitop||AA 679-751 Alternativen|
|2 Publikationen vorhanden|
|Hersteller||Anmelden zum Anzeigen|
ProduktdetailsAntigendetails Anwendungsinformationen Handhabung Referenzen Bilder
|Spezifität||Human Complement Component C5a / C5a|
|Produktmerkmale||Produced in rabbits immunized with purified, recombinant Human C5a (rh C5a, ABIN2003124 Leu 679-Arg 751,. C5a specific IgG was purified by human C5a affinity chromatography .|
|Reinigung||purified by affinity chromatography|
|Sterilität||0.2 μm filtered|
|Immunogen||Recombinant Human C5a protein (ABIN2003124)|
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|Hintergrund||C5a is a protein fragment released from complement component C5. This 74 amino acid peptide in humans is generated by the cleavage of C5a convertase on the C5 α-chain during the classical, alternative, and lectin pathways of complement activation. The structure of C5a includes a core region consisting of four, anti-parallel alpha-helices held together by three disulfide linkages and a structured C-terminal tail, and C5a is rapidly metabolised by carboxypeptidase B to a 73 amino acid low activity form, C5a des-Arg. C5a is an extremely potent proinflammatory mediator, as well as a potent chemotactic factor for neutrophils and other leukocytes. It causes histamine release, increases in vascular permeability, induces several cytokines production from leukocytes, enhances neutrophil-endothelial cell adhesion, and augments the humoral and cell-mediated immune response. C5a is quickly metabolised by carboxypeptidases, forming the less potent C5adesArg. Acting via a classical G protein-coupled receptor, CD88, C5a and C5adesArg exert a number of effects essential to the innate immune response, while their actions at the more recently discovered non-G protein-coupled receptor, C5L2 (or GPR77), remain unclear. The widespread expression of C5a receptors throughout the body allows C5a to elicit a broad range of effects. Thus, C5a has been found to be a significant pathogenic driver in a number of immuno-inflammatory diseases, making C5a inhibition an attractive therapeutic strategy. C5a is a strong chemoattractant and is involved in the recruitment of inflammatory cells such as neutrophils, eosinophils, monocytes, and T lymphocytes, in activation of phagocytic cells and release of granule-based enzymes and generation of oxidants, all of which may contribute to innate immune functions or tissue damage. Accordingly, the anaphylatoxin C5a is implicated in a variety of diseases such as rheumatoid arthritis, systemic lupus erythematosus, reperfusion injury, Alzheimer's disease, and sepsis.|
|Forschungsgebiet||Innate Immunity, Complement Factors|
|Pathways||Komplementsystem, Carbohydrate Homeostasis|
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ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human C5a. The detection limit for Human C5a is approximately 0.00975 ng/well.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
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|Buffer||0.2 μm filtered solution in PBS with 5 % trehalose|
|Handhabung||Avoid repeated freeze-thaw cycles.|
|Lagerung||4 °C,-20 °C,-80 °C|
|Informationen zur Lagerung||
This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
ReferenzenProduktdetails Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
Manthey, Woodruff, Taylor, Monk: "Complement component 5a (C5a)." in: The international journal of biochemistry & cell biology, Vol. 41, Issue 11, pp. 2114-7, 2009
Guo, Ward: "Role of C5a in inflammatory responses." in: Annual review of immunology, Vol. 23, pp. 821-52, 2005