PTGER2 ELISA Kit

Produktnummer ABIN5658141

Produktdetails PTGER2 ELISA Kit

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Prostaglandin E Receptor 2 (EP2). No significant cross-reactivity or interference between Prostaglandin E Receptor 2 (EP2) and analogues was observed.

Sensitivität: 29 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin E Receptor 2 (EP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin E Receptor 2 (EP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin E Receptor 2 (EP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin E Receptor 2 (EP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin E Receptor 2 (EP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin E Receptor 2 (EP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PTGER2

Target: PTGER2 (Prostaglandin E Receptor 2 (Subtype EP2), 53kDa (PTGER2))

Andere Bezeichnung: Prostaglandin E Receptor 2 (PTGER2 Produkte)

Synonyme: EP2 ELISA Kit, Ptgerep2 ELISA Kit, ptger2 ELISA Kit, ptger2l ELISA Kit, zgc:63974 ELISA Kit, MGC69060 ELISA Kit, PTGER2 ELISA Kit, Ptger-ep2 ELISA Kit, si:ch211-13n20.1 ELISA Kit, si:dkey-29m11.3 ELISA Kit, zgc:136608 ELISA Kit, prostaglandin E receptor 2 ELISA Kit, prostaglandin E receptor 2 (subtype EP2) ELISA Kit, prostaglandin E receptor 2a (subtype EP2) ELISA Kit, prostaglandin E receptor 2 S homeolog ELISA Kit, prostaglandin E receptor 2b (subtype EP2) ELISA Kit, PTGER2 ELISA Kit, Ptger2 ELISA Kit, ptger2a ELISA Kit, ptger2.S ELISA Kit, ptger2b ELISA Kit

Hintergrund:

Gene Name: Prostaglandin E Receptor 2

Gene Aliases: PTGER2, Prostaglandin E2 Receptor(Subtype EP2),53kD

Gen-ID: 5732

UniProt: P43116