PAM ELISA Kit

Produktnummer ABIN5657690

Produktdetails PAM ELISA Kit

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 31.25 pg/mL - 2000 pg/mL

Untere Nachweisgrenze: 31.25 pg/mL

Applikation: ELISA

Proben: Cerebrospinal Fluid, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Peptidylglycine Alpha Amidating Monooxygenase (PAM). No significant cross-reactivity or interference between Peptidylglycine Alpha Amidating Monooxygenase (PAM) and analogues was observed.

Sensitivität: 13.5 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidylglycine Alpha Amidating Monooxygenase (PAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidylglycine Alpha Amidating Monooxygenase (PAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidylglycine Alpha Amidating Monooxygenase (PAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidylglycine Alpha Amidating Monooxygenase (PAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PAM

Target: PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))

Andere Bezeichnung: Peptidylglycine Alpha Amidating Monooxygenase (PAM Produkte)

Synonyme: PAL ELISA Kit, PHM ELISA Kit, pal ELISA Kit, phm ELISA Kit, pamb ELISA Kit, CG3832 ELISA Kit, Dmel\\CG3832 ELISA Kit, dPHM ELISA Kit, AE-I ELISA Kit, PAL-A ELISA Kit, PHM-A ELISA Kit, pama ELISA Kit, si:dkeyp-35f12.2 ELISA Kit, peptidylglycine alpha-amidating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase S homeolog ELISA Kit, Peptidylglycine-alpha-hydroxylating monooxygenase ELISA Kit, peptidylglycine alpha-amidating monooxygenase L homeolog ELISA Kit, PAM ELISA Kit, Pam ELISA Kit, pam.S ELISA Kit, CHLREDRAFT_150435 ELISA Kit, sce7070 ELISA Kit, pam ELISA Kit, Phm ELISA Kit, pam.L ELISA Kit

Hintergrund:

Gene Name: Peptidylglycine Alpha Amidating Monooxygenase

Gene Aliases: PAL, PHM, Peptidylamidoglycolate lyase, Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase, Peptidylglycine Alpha-Hydroxylating Monooxygenase

Gen-ID: 5066

UniProt: P19021