PAD4 ELISA Kit
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- Target Alle PAD4 (PADI4) ELISA Kits anzeigen
- PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.312 ng/mL - 20 ng/mL
- Untere Nachweisgrenze
- 0.312 ng/mL
- Applikation
- ELISA
- Proben
- Plasma, Serum, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Peptidyl Arginine Deiminase Type IV (PADI4). No significant cross-reactivity or interference between Peptidyl Arginine Deiminase Type IV (PADI4) and analogues was observed.
- Sensitivität
- 0.121 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Arginine Deiminase Type IV (PADI4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Arginine Deiminase Type IV (PADI4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Arginine Deiminase Type IV (PADI4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Arginine Deiminase Type IV (PADI4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle PAD4 (PADI4) ELISA Kits anzeigen
- PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))
- Andere Bezeichnung
- Peptidyl Arginine Deiminase Type IV (PADI4 Produkte)
- Synonyme
- PAD ELISA Kit, PAD4 ELISA Kit, PADI5 ELISA Kit, PDI4 ELISA Kit, PDI5 ELISA Kit, Pad4 ELISA Kit, Pdi4 ELISA Kit, peptidyl arginine deiminase 4 ELISA Kit, peptidyl arginine deiminase, type IV ELISA Kit, PADI4 ELISA Kit, Padi4 ELISA Kit
- Hintergrund
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Gene Name: Peptidyl Arginine Deiminase Type IV
Gene Aliases: PAD, PADI5, PDI4, PDI5, Peptidyl Arginine Deiminase Type V, Peptidylarginine deiminase IV
- Gen-ID
- 23569
- UniProt
- Q9UM07
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