MFGE8 ELISA Kit

Produktnummer ABIN5656910

Produktdetails MFGE8 ELISA Kit

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.62 pg/mL - 1000 pg/mL

Untere Nachweisgrenze: 15.62 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Milk Fat Globule EGF Factor 8 (MFGE8). No significant cross-reactivity or interference between Milk Fat Globule EGF Factor 8 (MFGE8) and analogues was observed.

Sensitivität: 5.9 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Milk Fat Globule EGF Factor 8 (MFGE8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Milk Fat Globule EGF Factor 8 (MFGE8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Milk Fat Globule EGF Factor 8 (MFGE8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Milk Fat Globule EGF Factor 8 (MFGE8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: Milk Fat Globule EGF Factor 8 (MFGE8 Produkte)

Synonyme: BA46 ELISA Kit, EDIL1 ELISA Kit, HMFG ELISA Kit, HsT19888 ELISA Kit, MFG-E8 ELISA Kit, MFGM ELISA Kit, OAcGD3S ELISA Kit, SED1 ELISA Kit, SPAG10 ELISA Kit, hP47 ELISA Kit, ba46 ELISA Kit, edil1 ELISA Kit, oacgd3s ELISA Kit, MGC75581 ELISA Kit, hst19888 ELISA Kit, MGC145109 ELISA Kit, MFGE8 ELISA Kit, mfge8 ELISA Kit, zgc:154157 ELISA Kit, MGC99228 ELISA Kit, AA408458 ELISA Kit, AI325141 ELISA Kit, Mfgm ELISA Kit, P47 ELISA Kit, lactadherin ELISA Kit, AGS ELISA Kit, MFGME8 ELISA Kit, MFGMP-E8 ELISA Kit, im:6895406 ELISA Kit, mfge8l ELISA Kit, zgc:110846 ELISA Kit, milk fat globule-EGF factor 8 protein ELISA Kit, milk fat globule-EGF factor 8 protein b ELISA Kit, milk fat globule-EGF factor 8 protein L homeolog ELISA Kit, milk fat globule-EGF factor 8 protein a ELISA Kit, MFGE8 ELISA Kit, mfge8 ELISA Kit, mfge8b ELISA Kit, mfge8.L ELISA Kit, Mfge8 ELISA Kit, mfge8a ELISA Kit

Hintergrund:

Gene Name: Milk Fat Globule EGF Factor 8

Gene Aliases: HMFG, MFGM, SED1, BA46, EDIL1, OAcGD3S, SPAG10, hP47, Medin, Lactadherin, Sperm Associated Antigen 10, Sperm Surface Protein hP47, Milk fat globule-EGF factor 8

Gen-ID: 25277

UniProt: P70490

Pathways: SARS-CoV-2 Protein Interaktom