PNLIP ELISA Kit

Produktnummer ABIN5656445

Produktdetails PNLIP ELISA Kit

Target: PNLIP (Pancreatic Lipase (PNLIP))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Lipase, Pancreatic (PL). No significant cross-reactivity or interference between Lipase, Pancreatic (PL) and analogues was observed.

Sensitivität: 2.7 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Pancreatic (PL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase, Pancreatic (PL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase, Pancreatic (PL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lipase, Pancreatic (PL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lipase, Pancreatic (PL) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lipase, Pancreatic (PL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu PNLIP

Target: PNLIP (Pancreatic Lipase (PNLIP))

Andere Bezeichnung: Lipase, Pancreatic (PNLIP Produkte)

Synonyme: PL ELISA Kit, PNLIPD ELISA Kit, PTL ELISA Kit, LIPASE ELISA Kit, PANLI ELISA Kit, 1810007A24Rik ELISA Kit, PNLIP ELISA Kit, PNLIPRP2 ELISA Kit, pancreatic lipase ELISA Kit, PNLIP ELISA Kit, Pnlip ELISA Kit, pnlip ELISA Kit

Hintergrund:

Gene Name: Lipase, Pancreatic

Gene Aliases: PNLIP, PTL, Pancreatic Lipase, Pancreatic triacylglycerol lipase

Gen-ID: 69060

UniProt: Q6P8U6