HAVCR1 ELISA Kit

Produktnummer ABIN5656217

Produktdetails HAVCR1 ELISA Kit

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Untere Nachweisgrenze: 62.5 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Tissue Homogenate, Urine

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Kidney Injury Molecule 1 (Kim1). No significant cross-reactivity or interference between Kidney Injury Molecule 1 (Kim1) and analogues was observed.

Sensitivität: 23.3 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney Injury Molecule 1 (Kim1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney Injury Molecule 1 (Kim1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney Injury Molecule 1 (Kim1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kidney Injury Molecule 1 (Kim1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kidney Injury Molecule 1 (Kim1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu HAVCR1

Target: HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))

Andere Bezeichnung: Kidney Injury Molecule 1 (HAVCR1 Produkte)

Synonyme: HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit

Substanzklasse: Virus

Hintergrund:

Gene Name: Kidney Injury Molecule 1

Gene Aliases: HAVCR1, TIM1, TIMD1, HAVCR, Hepatitis A Virus Cellular Receptor 1, T Cell Immunoglobulin And Mucin Domain-Containing Protein 1, T-cell immunoglobulin mucin receptor 1

Gen-ID: 286934

UniProt: O54947