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IARS ELISA Kit

IARS Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5656124
  • Target Alle IARS ELISA Kits anzeigen
    IARS (Isoleucyl-tRNA Synthetase (IARS))
    Reaktivität
    • 3
    • 2
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Isoleucyl tRNA Synthetase (IARS). No significant cross-reactivity or interference between Isoleucyl tRNA Synthetase (IARS) and analogues was observed.
    Sensitivität
    0.114 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Isoleucyl tRNA Synthetase (IARS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Isoleucyl tRNA Synthetase (IARS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Isoleucyl tRNA Synthetase (IARS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Isoleucyl tRNA Synthetase (IARS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Isoleucyl tRNA Synthetase (IARS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle IARS ELISA Kits anzeigen
    IARS (Isoleucyl-tRNA Synthetase (IARS))
    Andere Bezeichnung
    Isoleucyl tRNA Synthetase (IARS Produkte)
    Synonyme
    fi46h05 ELISA Kit, zgc:63790 ELISA Kit, wu:fi46h05 ELISA Kit, K19E20.18 ELISA Kit, K19E20_18 ELISA Kit, ovule abortion 2 ELISA Kit, ECK0027 ELISA Kit, ilvS ELISA Kit, JW0024 ELISA Kit, An08g06770 ELISA Kit, AO090012000505 ELISA Kit, 2510016L12Rik ELISA Kit, AI327140 ELISA Kit, AU044614 ELISA Kit, E430001P04Rik ELISA Kit, ILRS ELISA Kit, Iarsl ELISA Kit, IARS1 ELISA Kit, ILERS ELISA Kit, IRS ELISA Kit, PRO0785 ELISA Kit, isoleucyl-tRNA synthetase ELISA Kit, tRNA synthetase class I (I, L, M and V) family protein ELISA Kit, isoleucine--tRNA ligase ELISA Kit, isoleucyl-tRNA synthetase, cytoplasmic ELISA Kit, isoleucine-tRNA synthetase ELISA Kit, iars ELISA Kit, IARS ELISA Kit, OVA2 ELISA Kit, ileS ELISA Kit, MBAR_RS19160 ELISA Kit, Rmag_0340 ELISA Kit, ANI_1_964074 ELISA Kit, CHLREDRAFT_106327 ELISA Kit, EDI_049880 ELISA Kit, OE_RS08520 ELISA Kit, AOR_1_1912194 ELISA Kit, Iars ELISA Kit
    Hintergrund

    Gene Name: Isoleucyl tRNA Synthetase

    Gene Aliases: IARS1, ILRS, OJ, IleRS, IRS, Isoleucine tRNA Ligase 1,Cytoplasmic

    Gen-ID
    3376
    UniProt
    P41252
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