CYP1A2 ELISA Kit
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- Target Alle CYP1A2 ELISA Kits anzeigen
- CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))
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Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.625 ng/mL - 40 ng/mL
- Untere Nachweisgrenze
- 0.625 ng/mL
- Applikation
- ELISA
- Proben
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A2 (CYP1A2). No significant cross-reactivity or interference between Cytochrome P450 1A2 (CYP1A2) and analogues was observed.
- Sensitivität
- 0.238 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A2 (CYP1A2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A2 (CYP1A2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A2 (CYP1A2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A2 (CYP1A2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A2 (CYP1A2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A2 (CYP1A2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle CYP1A2 ELISA Kits anzeigen
- CYP1A2 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 2 (CYP1A2))
- Andere Bezeichnung
- Cytochrome P450 1A2 (CYP1A2 Produkte)
- Synonyme
- CP12 ELISA Kit, P3-450 ELISA Kit, P450(PA) ELISA Kit, CYP1A2 ELISA Kit, CYPIA2 ELISA Kit, P450-PM4 ELISA Kit, Cyp1a1 ELISA Kit, P450-3 ELISA Kit, CYPD45 ELISA Kit, P-450d ELISA Kit, RATCYPD45 ELISA Kit, CYP1A5 ELISA Kit, CP450 ELISA Kit, CYP-IA2 ELISA Kit, cytochrome P450 family 1 subfamily A member 2 ELISA Kit, cytochrome P450 1A2 ELISA Kit, cytochrome P450 LM4 ELISA Kit, cytochrome P450, family 1, subfamily A, polypeptide 2 ELISA Kit, cytochrome P450, family 1, subfamily a, polypeptide 2 ELISA Kit, cytochrome P450 1A2-like ELISA Kit, CYP1A2 ELISA Kit, LOC100328937 ELISA Kit, Cyp1a2 ELISA Kit, VDBG_06455 ELISA Kit, LOC101825565 ELISA Kit, LOC102130662 ELISA Kit
- Hintergrund
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Gene Name: Cytochrome P450 1A2
Gene Aliases: CP12, P3-450, P450(PA), Cytochrome P450,Family 1,Subfamily A,Polypeptide 2
- Gen-ID
- 1544
- UniProt
- P05177
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