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CDKN3 ELISA Kit

CDKN3 Reaktivität: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Produktnummer ABIN5653431
  • Target Alle CDKN3 ELISA Kits anzeigen
    CDKN3 (Cyclin-Dependent Kinase Inhibitor 3 (CDKN3))
    Reaktivität
    • 3
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.156 ng/mL - 10 ng/mL
    Untere Nachweisgrenze
    0.156 ng/mL
    Applikation
    ELISA
    Proben
    Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Cyclin Dependent Kinase Inhibitor 3 (CDKN3). No significant cross-reactivity or interference between Cyclin Dependent Kinase Inhibitor 3 (CDKN3) and analogues was observed.
    Sensitivität
    0.053 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin Dependent Kinase Inhibitor 3 (CDKN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin Dependent Kinase Inhibitor 3 (CDKN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin Dependent Kinase Inhibitor 3 (CDKN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 3 (CDKN3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin Dependent Kinase Inhibitor 3 (CDKN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle CDKN3 ELISA Kits anzeigen
    CDKN3 (Cyclin-Dependent Kinase Inhibitor 3 (CDKN3))
    Andere Bezeichnung
    Cyclin Dependent Kinase Inhibitor 3 (CDKN3 Produkte)
    Synonyme
    CDI1 ELISA Kit, CIP2 ELISA Kit, KAP ELISA Kit, KAP1 ELISA Kit, CDKN3 ELISA Kit, 2410006H10Rik ELISA Kit, K24G6.15 ELISA Kit, K24G6_15 ELISA Kit, KIP-RELATED PROTEIN 3 ELISA Kit, KRP3 ELISA Kit, inhibitor/interactor with cyclin-dependent kinase ELISA Kit, cyclin dependent kinase inhibitor 3 ELISA Kit, cyclin-dependent kinase inhibitor 3 ELISA Kit, inhibitor/interactor with cyclin-dependent kinase ELISA Kit, CDKN3 ELISA Kit, cdkn3 ELISA Kit, Cdkn3 ELISA Kit, ICK6 ELISA Kit
    Hintergrund

    Gene Name: Cyclin Dependent Kinase Inhibitor 3

    Gene Aliases: CDI1, CIP2, KAP1, CDK2-Associated Dual Specificity Phosphatase, Kinase Associated Phosphatase, Cyclin-dependent kinase interactor 1, Cyclin-dependent kinase-interacting 2

    Gen-ID
    1033
    UniProt
    Q16667
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