Osteoprotegerin ELISA Kit
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- Target Alle Osteoprotegerin (TNFRSF11B) ELISA Kits anzeigen
- Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
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Reaktivität
- Schwein
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.1-30 ng/mL
- Untere Nachweisgrenze
- 0.1 ng/mL
- Applikation
- ELISA
- Verwendungszweck
- Porcine Osteoprotegerin (TNFRSF11B) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Proben
- Plasma, Cell Culture Supernatant, Serum
- Analytische Methode
- Quantitative
- Spezifität
- The antibody pair provided in this kit recognizes Porcine Osteoprotegerin (TNFRSF11B)
- Sensitivität
- 0.12 ng/mL
- Produktmerkmale
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Bestandteile
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Benötigtes Material
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Applikationshinweise
- Recommended Dilution for serum and plasma samples2 fold
- Probenmenge
- 100 μL
- Plattentyp
- Pre-coated
- Protokoll
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Testdurchführung
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Lagerung
- -20 °C
- Informationen zur Lagerung
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Haltbarkeit
- 6 months
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- Target Alle Osteoprotegerin (TNFRSF11B) ELISA Kits anzeigen
- Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
- Andere Bezeichnung
- Osteoprotegerin (TNFRSF11B Produkte)
- Synonyme
- CD254 ELISA Kit, ODF ELISA Kit, OPGL ELISA Kit, OPTB2 ELISA Kit, RANKL ELISA Kit, TRANCE ELISA Kit, hRANKL2 ELISA Kit, sOdf ELISA Kit, OCIF ELISA Kit, OPG ELISA Kit, TR1 ELISA Kit, Opg ELISA Kit, ocif ELISA Kit, opg ELISA Kit, tr1 ELISA Kit, TNF superfamily member 11 ELISA Kit, TNF receptor superfamily member 11b ELISA Kit, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) ELISA Kit, TNF receptor superfamily member 11B ELISA Kit, tumor necrosis factor receptor superfamily member 11b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily, member 11b ELISA Kit, TNFSF11 ELISA Kit, TNFRSF11B ELISA Kit, Tnfrsf11b ELISA Kit, tnfrsf11b.S ELISA Kit
- Substanzklasse
- Chemical
- Hintergrund
- Osteoprotegerin (TNFRSF11B)
- Gen-ID
- 100738370
- UniProt
- F1S278
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