FITC Annexin V Apoptosis Detection Kit with 7-AAD

Details zu Produkt Nr. ABIN2669212, Anbieter: Anmelden zum Anzeigen
Antigen
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
  • annexin A5
  • annexin A5b
  • Annexin A5
  • annexin A5 L homeolog
  • ANXA5
  • anxa5b
  • anxa5
  • Anxa5
  • anxa5.L
Alternativen
Human Annexin V ELISA Kit
Reaktivität
Säugetier
18
15
15
14
13
8
7
7
4
2
2
2
2
1
1
1
1
Konjugat
FITC
Applikation
Multiplex Assay (MA)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Produktmerkmale FITC Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our 7-amino-actinomycin D (7-AAD) solution. Early apoptotic cells will exclude 7-AAD, while late stage apoptotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. 7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
Bestandteile FITC Annexin V Apoptosis Detection Kit with 7-AAD
Andere Bezeichnung Annexin V (ANXA5 ELISA Kit Abstract)
Forschungsgebiet Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Vorsichtsmaßnahmen Caution: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store between 2°C and 8°C.
Bilder des Herstellers
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with 7-AAD (ABIN2669212) FITC Annexin V Apoptosis Detection Kit with 7-AAD
Flow Cytometry (FACS) image for FITC Annexin V Apoptosis Detection Kit with 7-AAD (ABIN2669212) FITC Annexin V Apoptosis Detection Kit with 7-AAD (Image 2)
Allgemeine Veröffentlichungen Maciel, Neves, Santinha, Reis, Domingues, Teresa Cruz, Pitt, Spickett, Domingues: "Detection of phosphatidylserine with a modified polar head group in human keratinocytes exposed to the radical generator AAPH." in: Archives of biochemistry and biophysics, Vol. 548, pp. 38-45, 2014 (PubMed).