MST1 ELISA Kit (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like))

Details for Product MST1 ELISA Kit No. ABIN1889321, Anbieter: Anmelden zum Anzeigen
Antigen
  • D3F15S2
  • DNF15S2
  • HGFL
  • MSP
  • NF15S2
  • D3F15S2h
  • D9H3F15S2
  • DNF15S2h
  • Hgfl
  • E2F2
  • HGF1/MSP
  • macrophage stimulating 1 (hepatocyte growth factor-like)
  • Macrophage stimulating 1 (hepatocyte growth factor-like)
  • MST1
  • Mst1
Epitop
AA 19-711
Reaktivität
Human
Alternativen
Kits mit alternativen Reaktivitäten:
12
11
5
2
2
2
2
2
1
1
1
Methodentyp
Sandwich ELISA
Detektionsbereich
156-10000 pg/mL
Untere Nachweisgrenze
156 pg/mL
Applikation
ELISA
Optionen
Hersteller
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Verwendungszweck Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MSP/MST1
Marke PicoKine™
Proben Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
Analytische Methode Quantitative
Nachweismethode Colorimetric
Immunogen Expression system for standard: NSO
Immunogen sequence: Q19-G711
Spezifität Expression system for standard: NSO
Immunogen sequence: Q19-G711
Kreuzreaktivität (Details) There is no detectable cross-reactivity with other relevant proteins.
Sensitivität <10pg/mL
Benötigtes Material Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
Andere Bezeichnung MST1 (MST1 ELISA Kit Abstract)
Hintergrund

Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Synonyms: Hepatocyte growth factor-like protein,Macrophage stimulatory protein,Macrophage-stimulating protein,MSP,Hepatocyte growth factor-like protein alpha chain,Hepatocyte growth factor-like protein beta chain,MST1,D3F15S2, DNF15S2, HGFL,

Full Gene Name: Hepatocyte growth factor-like protein

Cellular Localisation: Secreted.
Gen-ID 4485
UniProt P26927
Forschungsgebiet Cancer, Innate Immunity, Signaling
Applikationshinweise Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
Kommentare

Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

Plattentyp Pre-coated
Protokoll human MSP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MSP has been precoated onto 96-well plates. Standards(NSO, Q19-G711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MSP amount of sample captured in plate.
Testdurchführung

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MSP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MSP standard solution and each sample be measured in duplicate.

Testpräzision
  • Sample 1: n=16, Mean(pg/ml): 1126, Standard deviation: 56.3, CV(%): 5
  • Sample 2: n=16, Mean(pg/ml): 2738, Standard deviation: 170, CV(%): 6.2
  • Sample 3: n=16, Mean(pg/ml): 5273, Standard deviation: 242.6, CV(%): 4.6,
  • Sample 1: n=24, Mean(pg/ml): 1384, Standard deviation: 78.9, CV(%): 5.7
  • Sample 2: n=24, Mean(pg/ml): 2941, Standard deviation: 206, CV(%): 7
  • Sample 3: n=24, Mean(pg/ml): 5527, Standard deviation: 364.8, CV(%): 6.6
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Avoid multiple freeze-thaw cycles.
Lagerung -20 °C,4 °C
Informationen zur Lagerung Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
Haltbarkeit 12 months
Bilder des Herstellers
ELISA image for MST1 ELISA Kit (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like)) (ABIN1889321) Human MSP/MST1 PicoKine ELISA Kit standard curve
Allgemeine Veröffentlichungen Sakamoto, Iwama, Amitani, Takehara, Yamaguchi, Yamamoto, Masuyama, Yamanaka, Ando, Suda: "Role of macrophage-stimulating protein and its receptor, RON tyrosine kinase, in ciliary motility." in: The Journal of clinical investigation, Vol. 99, Issue 4, pp. 701-9, 1997 (PubMed).

Han, Stuart, Degen: "Characterization of the DNF15S2 locus on human chromosome 3: identification of a gene coding for four kringle domains with homology to hepatocyte growth factor." in: Biochemistry, Vol. 30, Issue 40, pp. 9768-80, 1991 (PubMed).

Gerber, Drabkin, Firnhaber, Miller, Scoggin, Smith: "Regional localization of chromosome 3-specific DNA fragments by using a hybrid cell deletion mapping panel." in: American journal of human genetics, Vol. 43, Issue 4, pp. 442-51, 1988 (PubMed).