CCL1 ELISA Kit

Produktnummer ABIN1889303

Produktdetails CCL1 ELISA Kit

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Bindungsspezifität: AA 24-92

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL1/TCA3

Marke: PicoKine™

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NSO
Immunogen sequence: K24-C92

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: <1pg/mL

Benötigtes Material: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: K24-C92

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plattentyp: Pre-coated

Protokoll: mouse CCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL1 has been precoated onto 96-well plates. Standards(NSO, K24-C92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL1 amount of sample captured in plate.

Testdurchführung:

Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL1 standard solution and each sample is measured in duplicate.

Testpräzision:

• Sample 1: n=16, Mean(pg/ml): 34, Standard deviation: 1.5, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 121, Standard deviation: 6.9, CV(%): 5.7
• Sample 3: n=16, Mean(pg/ml): 389, Standard deviation: 25.7, CV(%): 6.6,
• Sample 1: n=24, Mean(pg/ml): 35, Standard deviation: 2.2, CV(%): 6.3
• Sample 2: n=24, Mean(pg/ml): 127, Standard deviation: 8.6, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 403, Standard deviation: 32.6, CV(%): 8.1

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Haltbarkeit: 12 months

Referenzen für CCL1 ELISA Kit (ABIN1889303)

Wu, Chen: "Extracted Triterpenes from Antrodia cinnamomea Reduce the Inflammation to Promote the Wound Healing via the STZ Inducing Hyperglycemia-Diabetes Mice Model." in: Frontiers in pharmacology, Vol. 7, pp. 154, (2016) (PubMed).

Details zu CCL1

Target: CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

Andere Bezeichnung: CCL1 (CCL1 Produkte)

Synonyme: I-309 ELISA Kit, P500 ELISA Kit, SCYA1 ELISA Kit, SISe ELISA Kit, TCA3 ELISA Kit, BF534335 ELISA Kit, Scya1 ELISA Kit, Tca-3 ELISA Kit, CCLi6 ELISA Kit, chemokine ELISA Kit, C-C motif chemokine ligand 1 ELISA Kit, chemokine (C-C motif) ligand 1 ELISA Kit, CCL1 ELISA Kit, Ccl1 ELISA Kit

Hintergrund:

Protein Function: Cytokine that is chemotactic for neutrophils.

Background: CCL1, Chemokine(C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Synonyms: C-C motif chemokine 1,P500,SIS-epsilon,Small-inducible cytokine A1,T-cell activation protein 3,TCA-3,TCA3,Ccl1,Scya1, Tca3,

Full Gene Name: C-C motif chemokine 1

Gen-ID: 20290

UniProt: P10146