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|Antigen||Interferon alpha (IFNA) ELISA Kits|
Kits mit alternativen Reaktivitäten:
|Detektionsbereich||7.8 pg/mL - 500 pg/mL|
|Untere Nachweisgrenze||7.8 pg/mL|
|7 Publikationen vorhanden|
|Hersteller||Anmelden zum Anzeigen|
Produktdetails IFNA ELISA KitAntigendetails Anwendungsinformationen Handhabung Referenzen für IFNA Kit (ABIN414421) Bilder
|Verwendungszweck||The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IFNa in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids|
|Proben||Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids|
This assay has high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa).
|Kreuzreaktivität (Details)||No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.|
AntigendetailsProduktdetails IFNA ELISA Kit Anwendungsinformationen Handhabung Referenzen für IFNA Kit (ABIN414421) Bilder zurück nach oben
|Andere Bezeichnung||IFNa (IFNA ELISA Kit Abstract)|
|Pathways||JAK-STAT Signalweg, Hepatitis C|
AnwendungsinformationenProduktdetails IFNA ELISA Kit Antigendetails Handhabung Referenzen für IFNA Kit (ABIN414421) Bilder zurück nach oben
Information on standard material:
|Protokoll||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Alpha (IFNa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Alpha (IFNa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.|
|Aufbereitung der Reagenzien||
Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C
Cell Lysate: Cells must be lysed before assaying according to the following directions. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). Wash cells three times in cold PBS. Resuspend cells in PBS (1×) and subject them to ultrasonication for 4 times (or Freeze cells at -20 °C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle for 3 times.) Centrifuge at 1500 × g for 10 minutes at 2 - 8°C to remove cellular debris.
Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
|Aufbereitung der Proben||
Average the duplicate readings for each standard, control, and samples and subtract the average zero standard optical density. Create a standard curve on log-log graph paper, with IFNa concentration on the y-axis and absorbance on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, for instance, curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), plotting log of the data to establish standard curve for each test is recommended. Typical standard curve below is provided for reference only.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
|Beschränkungen||Nur für Forschungszwecke einsetzbar|
HandhabungProduktdetails IFNA ELISA Kit Antigendetails Anwendungsinformationen Referenzen für IFNA Kit (ABIN414421) Bilder zurück nach oben
|Vorsichtsmaßnahmen||The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.|
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
|Informationen zur Lagerung||
Referenzen für IFNA Kit (ABIN414421)Produktdetails IFNA ELISA Kit Antigendetails Anwendungsinformationen Handhabung Bilder zurück nach oben
|Produkt verwendet in:||
Yu, Li, Cheng, Huang, Zheng, Chen, Ling, Zhu, Chen, Shi, Li: "MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B." in: Biochemical and biophysical research communications, Vol. 488, Issue 4, pp. 628-633, 2017
Liu, Qin, Kong, Shao, Su, Wang, Chen: "siRNA Targeting the 2Apro Genomic Region Prevents Enterovirus 71 Replication In Vitro." in: PLoS ONE, Vol. 11, Issue 2, pp. e0149470, 2016
Yu, Chen, Tseng, Lin, Hsu, Chen, Lee: "Sulforaphane Suppresses Hepatitis C Virus Replication by Up-Regulating Heme Oxygenase-1 Expression through PI3K/Nrf2 Pathway." in: PLoS ONE, Vol. 11, Issue 3, pp. e0152236, 2016
Hoffmann, Bruns, Bäckdahl, Neregård, Niederreiter, Herrmann, Catrina, Agerberth, Holmdahl: "The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats." in: Annals of the rheumatic diseases, Vol. 72, Issue 7, pp. 1239-48, 2013
Okazaki, Nagoya, Tateda, Katada, Mizuo, Watanabe, Yamashita, Matsumoto: "Experimental rat model for alcohol-induced osteonecrosis of the femoral head." in: International journal of experimental pathology, Vol. 94, Issue 5, pp. 312-9, 2013
Lee, Tseng, Young, Sun, Wang, Chen, Lin, Wu: "Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells." in: British journal of pharmacology, Vol. 171, Issue 1, pp. 237-52, 2013
Suleiman, Nashef, Moussa, el-Islam: "Psychosocial profile of the parasuicidal patient in Kuwait." in: The International journal of social psychiatry, Vol. 32, Issue 3, pp. 16-22, 1986
BilderProduktdetails IFNA ELISA Kit Antigendetails Anwendungsinformationen Handhabung Referenzen für IFNA Kit (ABIN414421) zurück nach oben
|Bilder des Herstellers||