anti-Ratte (Rattus) CSF2RA Antikörper für Western Blotting

Recommended CSF2RA Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) Antikörper
  • CD116
  • CDw116
  • CSF2R
  • CSF2RAX
  • CSF2RAY
  • CSF2RX
  • CSF2RY
  • GM-CSF-R-alpha
  • GMCSFR
  • GMR
  • SMDP4
  • Csfgmra
  • GM-CSF-Ra
  • GM-CSFR
  • colony stimulating factor 2 receptor, alpha
  • colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage)
  • CSF2RA
  • Csf2ra
Epitop
Center
18
12
11
10
6
5
5
5
5
4
3
3
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
163
33
7
Wirt
Kaninchen
90
80
16
2
Klonalität
Polyklonal
Konjugat
Dieser CSF2RA Antikörper ist unkonjugiert
16
11
11
6
6
5
5
5
4
4
4
4
3
2
2
2
2
2
2
2
2
2
1
Applikation
Western Blotting (WB)
104
104
49
26
15
8
4
3
3
3
2
2
2
2
2
2
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

Produktnummer ABIN2705734
464,29 €
Zzgl. Versandkosten 20,00 € und MWSt
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1 ABIN2740128 WB Rabbit Internal Region Anmelden zum Anzeigen Polyclonal
1 ABIN2894377 IHC WB Rabbit Anmelden zum Anzeigen Polyclonal
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General

Antigen Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) Antikörper
Epitop Center
(18), (12), (11), (10), (6), (5), (5), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1)
Reaktivität Human, Maus, Ratte (Rattus)
(163), (33), (7)
Wirt Kaninchen
(90), (80), (16), (2)
Klonalität
Polyklonal
Konjugat Dieser CSF2RA Antikörper ist unkonjugiert
(16), (11), (11), (6), (6), (5), (5), (5), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Applikation Western Blotting (WB)
(104), (104), (49), (26), (15), (8), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Hersteller Anmelden zum Anzeigen

Produktdetails anti-CSF2RA Antikörper

Target Details CSF2RA Anwendungsinformationen Handhabung Bilder
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN2711562.
Spezifität Recognizes endogenous levels of CD116 protein.
Produktmerkmale Rabbit polyclonal antibody to CD116
Reinigung The antibody was purified by immunogen affinity chromatography.
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD116.

Target Details CSF2RA

Produktdetails anti-CSF2RA Antikörper Anwendungsinformationen Handhabung Bilder zurück nach oben
Antigen
Andere Bezeichnung CD116 (CSF2RA Antibody Abstract)
Hintergrund CSF2R, CSF2RY, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116, CD116
Gen-ID 1438, 12982
UniProt P15509, Q00941
Forschungsgebiet CD Antigens, Surface Receptors of Immune Cells
Pathways JAK-STAT Signalweg

Anwendungsinformationen

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Handhabung Bilder zurück nach oben
Applikationshinweise WB (1:500 - 1:1000)
Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Anwendungsinformationen Bilder zurück nach oben
Format Liquid
Buffer Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Haltbarkeit 12 months

Bilder

Produktdetails anti-CSF2RA Antikörper Target Details CSF2RA Anwendungsinformationen Handhabung zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-CSF2RA Antikörper (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage)) (Center) (ABIN2705734) Western blot analysis of CD116 expression in HEK293T (A), Raw264.7 (B), H9C2 (C) whol...
'Independent Validation' Siegel
Antigen CSF2RA
Validierte Anwendung Western Blotting
Positivkontrolle primary mouse microglia
Negativkontrolle siRNA knock-down of CSF2RA in primary mouse microglia
Bewertung Passed. ABIN2705734 specifically recognizes Csf2ra in primary mouse microglia lysates.
Primärantikörper ABIN2705734
Sekundärantikörper anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
Protokoll
  • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
  • Transfect cells with 0.05nmol mouse CSF2RA Silencer Select siRNA (ThermoFisher Scientific, ASO1094M, ASO10991 and ASO109DF, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
  • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
  • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
  • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
  • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
  • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
  • Incubation with primary rabbit anti-CSF2RA antibody (antibodies-online, ABIN2705734, lot CP1D10A) diluted 1:500 in TBST for 1h at RT.
  • Wash membrane 5x 5min with TBST.
  • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
  • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
  • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
  • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
  • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
Anmerkungen The RND3 antibody ABIN2705734 reveals a protein of the expected molecular weight of mouse Csf2ra in lysates of primary mouse microglia. The protein band’s intensity decreases upon knockdown with two of the three utilized siRNAs.
Validierungsbilder
Western Blotting validation image for anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (Center) antibody (ABIN2705734) Primary mouse microglia were transfected with Csf2ra siRNA and Csf2ra was revealed us...