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Maus IgG1 isotype control (Biotin)

Details zu Produkt Nr. ABIN810078, Anbieter: Anmelden zum Anzeigen
Isotyp
  • IgG1
  • Igh-4
  • VH7183
Wirt
Maus
236
97
22
5
3
3
2
2
1
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Biotin
28
24
22
16
7
6
6
5
4
4
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Isotype Control (IsoC), Negative Control (NC), Western Blotting (WB)
Hersteller
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Klon MOPC-21
Isotyp IgG1
Spezifität This mouse IgG1 kappa monoclonal antibody (clone MOPC-21) has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
Keine Kreuzreaktivität Human, Ratte (Rattus)
Produktmerkmale The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 µg/mL ) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Reinigung Purified from ascites by protein-A affinity chromatography.
Gen-ID 3690
Forschungsgebiet Secondary Antibodies
Applikationshinweise Working concentrations should be determined by the investigator.I t is recommended that the user titrates the reagent for use in the particular testing system.

Working concentrations should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Produkt verwendet in: Rebetz, Tian, Persson et al.: "Glial progenitor-like phenotype in low-grade glioma and enhanced CD133-expression and neuronal lineage differentiation potential in high-grade glioma." in: PLoS ONE, Vol. 3, Issue 4, pp. e1936, 2008 (PubMed).

Smed-Sörensen, Moll, Cheng et al.: "IgG regulates the CD1 expression profile and lipid antigen-presenting function in human dendritic cells via FcgammaRIIa." in: Blood, Vol. 111, Issue 10, pp. 5037-46, 2008 (PubMed).

Yates, Rovis, Mitchell et al.: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, 2007 (PubMed).

Carlsten, Björkström, Norell et al.: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, 2007 (PubMed).

Bryceson, March, Barber et al.: "Cytolytic granule polarization and degranulation controlled by different receptors in resting NK cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 1001-12, 2005 (PubMed).

Wiendl, Mitsdoerffer, Schneider et al.: "Muscle fibres and cultured muscle cells express the B7.1/2-related inducible co-stimulatory molecule, ICOSL: implications for the pathogenesis of inflammatory myopathies." in: Brain : a journal of neurology, Vol. 126, Issue Pt 5, pp. 1026-35, 2003 (PubMed).